J
Jeffrey W. Williams
Researcher at University of Wisconsin-Madison
Publications - 9
Citations - 1122
Jeffrey W. Williams is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: Dihydrofolate reductase & Sulfhydryl reagent. The author has an hindex of 9, co-authored 9 publications receiving 1101 citations.
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Book ChapterDOI
The kinetics of reversible tight-binding inhibition.
Journal ArticleDOI
Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase.
TL;DR: Investigations of the slow, tight-binding inhibition by methotrexate of the reaction catalyzed by dihydrofolate reductase from Streptococcus faecium A show that progress curve data are in accord with a mechanism that involves the rapid formation of an enzyme-NADPH-methotrexate complex that subsequently undergoes a relatively slow, reversible isomerization reaction.
Journal ArticleDOI
Mercuric reductase from R-plasmid NR1: characterization and mechanistic study
Journal ArticleDOI
Synthesis of a tight-binding, multisubstrate analog inhibitor of gentamicin acetyltransferase I.
TL;DR: The effectiveness of the tight-binding between the enzyme and the multisubstrate analog demonstrates that inhibitors of resistance can be designed and prepared by specific enzymatic synthesis.
Journal ArticleDOI
Purification and properties of gentamicin acetyltransferase I.
TL;DR: The purified enzyme appears homogeneous by six criteria, the first aminoglycoside inactivating enzyme so purified, and is stable to a variety of conditions, including lyophilization and prolonged storage, and can be monitored by two convenient spectrophotometric assays.