The invention discloses a method for preparing a wormwood dye. The wormwood dye is prepared by the following steps: grinding wormwoods, performing ultrasonic-microwave assisted extraction, filtering, ceramic membrane purification, organic membrane concentration and spray drying, thereby obtaining the wormwood dye. According to the method disclosed by the invention, the extraction speed and extraction rate of plant dyes are improved, the time is saved, the energy consumption is reduced, and the method is suitable for industrial production. Moreover, woolen textiles dyed by the wormwood dye prepared by the method have antibacterial property of 95% or higher on staphylococcus aureus, antibacterial property of 98% or higher on Escherichia coli and antibacterial property of 90% or higher on Candida albicans.

Wormwood dye as well as preparation method and application thereof

The invention discloses a method for extraction of chlorophyll from safflower seedlings The method includes: conducting root removal operation on fresh safflower seedlings, in a protective atmosphere of inert gas, squeezing the root removed fresh safflower seedlings by vacuum negative pressure method to obtain juice and residual, filtering the juice, combining the filter residue obtained by filtration and the residual to obtain a to-be-extracted material; extracting the to-be-extracted material by soaking and microwave assisted ultrasonic wave extraction method to obtain a filtered liquid; filtering the filtered liquid, adsorbing the filtered liquid with macroporous resin, and collecting chlorophyll-rich eluent; and carrying out centrifugation, separation and concentration as well as three-stage reverse osmosis concentration on the chlorophyll-rich eluent to obtain an extract, and drying the extract by lyophilization, thus obtaining chlorophyll powder The method for extraction of chlorophyll from safflower seedlings provided by the invention has the advantages of high yield, low cost, low energy consumption, high purity and good color

Method for extraction of chlorophyll from safflower seedlings

The invention provides a process for extracting lonicera caerulea anthocyanins through acidized ethanol under cooperative assistance of ultrasonic waves-microwaves in order to solve the problem that the extraction efficiency of lonicera caerulea anthocyanins is low. The process comprises the specific steps of a, pretreatment of a raw material; b, mixing of acidized ethanol and the raw material; c, ultrasonic wave-microwave cooperative treatment; d, separation; e, constant volume obtaining; f, light absorption value measuring. According to the process, four variables, namely ethanol concentration, treatment time, microwave power and the material-to-liquid ratio are set; design expert software is utilized for designing and optimizing the optimal extraction process for lonicera caerulea powder according to Box-Behnken, wherein the optimal extraction process includes that the ethanol concentration is 58.51%, the treatment time is 3.21 min, the microwave power is 287.08 W, the material-to-liquid ratio is 1:59.60 g/mL, and the anthocyanin content is 33.5887 mg/g. Lonicera caerulea produced on the Changbai Mountain is used as the raw material, 0.1%(v/v) hydrochloric ethanol is used as an extracting agent, ultrasonic wave-microwave cooperative treatment is conducted, extraction time can be greatly shortened, the anthocyanin content is effectively increased, a new method is provided for extraction of lonicera caerulea anthocyanins, and the process has high application value and broad development prospects.

Process for extracting lonicera caerulea anthocyanins through acidized ethanol under cooperative assistance of ultrasonic waves-microwaves

Belonging to the technical field of natural product extraction, the invention provides a method for removal of saccharide from anthocyanin with a nanofiltration membrane. The method utilizes modifiedor unmodified polyamide membrane, polyimide membrane, polysulfone membrane or polyethersulfone membrane as the nanofiltration membrane to remove fructose and glucose from anthocyanin, wherein the saccharide removal rate reaches 30-60%, and anthocyanin basically has no loss, the method is simple and easy to operate, and effectively solves the problems of long process and low anthocyanin content after concentration in the traditional technology.

Method for removal of saccharide from anthocyanin with nanofiltration membrane

The invention discloses a method and device for extracting anthocyanins from black rice. The method includes peeling a black rice raw material twice, sieving the black rice raw material once, mixing afirst-grade powder peel material, a second-grade powder peel material and a powder material collected by a vibrating sieve uniformly, and pouring the mixture into a black rice peel digestion device quantitatively; adding pure water to the black rice peel digestion device to prepare slurry, adding enzyme to the slurry, adding citric acid, increasing the temperature to 45-50 DEG C, standing for heat insulation, then adding poloxamer and anionic polyacrylamide, stirring till completely dissolving and finally sieving with a 200 mesh sieve to obtain a filtrate that is an extract; conducting ultrafiltration and nanofiltration on the extract, adding the extract into a separation column filled with macroporous adsorption resin, then adding the wet macroporous adsorption resin to 2-3 times by weight of a 75% ethanol solution after no solution flows out at the bottom, removing the macroporous adsorption resin by sieving to obtain a separation solution; introducing the separation solution into aspray dryer, pulverizing dried particles through an ultrafine pulverizer to obtain the black rice anthocyanins.

Method for extracting anthocyanins from black rice

The invention provides a black rice anthocyanin extracting method. The black rice anthocyanin extracting method specifically comprises the following steps: taking a black rice grain as a material, preprocessing the black rice grain, coarsely extracting the anthocyanin in the black rice grain, and then sequentially perform ultrafiltration, nanofiltration, macroporous resin adsorption, ethyl alcohol elution, secondary nanofiltration, and freeze-drying in vacuum to obtain a freeze-dried powder of the black rice anthocyanin. The black rice anthocyanin extracting method provided by the invention is good in selectivity; impurities of polysaccharide, organic acids, proteins and the like in the black rice anthocyanin can be effectively separated; the extracting rate is high, the product quality is high, even trace of the black rice anthocyanin is also extracted, the extracting speed is high, the operation is simple, and the discharge of effluent is low.

Black rice anthocyanin extracting method

The invention discloses a method for extracting and detecting heme from plants. The method for extracting and detecting the heme from the plants comprises the following steps of 1 adopting a mixed solution prepared by acetone and distilled water to perform centrifugal separation so as to remove chlorophyll, then using mixed solution prepared by hydrochloric acid, acetone and distilled water to perform centrifugal separation so as to obtain a mixed extracting solution, then using diethyl ether or perform extraction, and performing concentration and drying to obtain dried plant heme; 2 adopting an ultraviolet spectrophotometer to detect absorbancy at the 574.5 nm position; 3 substituting average absorbancy A obtained in the step 2 into a linear regression equation: A=2.6814496+0.07506221 to obtain the dilution ratio C through calculation, and then calculating the concentration Ci of the heme in plant leaves according to Ci=0.5Cmg/m1. By means of the method for extracting and detecting the heme from the plants, the heme content in the plants can be accurately detected, and the method plays an important role on research on a chlorophyll metabolism regulatory mechanism.

Method for extracting and detecting heme from plants

The invention discloses an extraction method of purple corn anthocyanin. The method comprises the following steps: by using purple corn grains as the raw material, carrying out pretreatment, carrying out crude extraction on anthocyanin in the purple corn grains, sequentially carrying out ultrafiltration, nanofiltration, macroporous resin adsorption, ethanol elution and secondary nanofiltration, and carrying out vacuum freeze drying to obtain the purple corn anthocyanin. The extraction method provided by the invention has favorable selectivity, can effectively separate polysaccharides, organic acids, proteins and other impurities in the purple corn anthocyanin, has the advantages of high extraction rate, high product quality, high extraction speed and small waste liquid discharge amount, and can extract a trace amount of anthocyanin.

Jia Qishi

Papers

Black rice anthocyanin extracting method

Method for extracting and detecting heme from plants

Extraction method of purple corn anthocyanin