Showing papers by "Jian Ni published in 2012"

Simultaneous determination and pharmacokinetic study of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS-MS.

Abstract: For the first time, a rapid and specific LC-MS-MS method has been developed for the analysis of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII in beagle dog plasma. The method was applied to study the pharmacokinetics of Rhizoma Paridis extracts containing polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII. The analysis was carried out on an Agilent Zorbax XDB-C(18) reversed-phase column (100 × 2.1 mm, 1.8 µm) by isocratic elution with acetonitrile and water (50:50, v/v). The flow rate was 0.25 mL/min. All analytes including internal standards were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII ranging from 10 to 5000 ng/mL. The intra-and inter-day precisions (RSDs) were less than 6.66 and 9.15%. The extraction recovery ranged from 95.53 to 104.21% with RSD less than 8.69%. Stability studies showed that polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII were stable in preparation and analytical process. The validated method was successfully used to determine the concentration-time profiles of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII.

Simultaneous determination of Polyphyllin I and Polyphyllin II from Rhyzoma Paridis dog plasma by a rapid HPLC and its application to pharmacokinetic study after oral administration

Abstract: For the first time, a rapid and specific reversed phase-high performance liquid chromatography-ultraviolet (RP-HPLC-UV) method was developed for the analysis of polyphyllin I and polyphyllin II in beagle dog plasma The method was applied to study the pharmacokinetics of Rhizoma paridis capsule containing polyphyllin I and polyphyllin II The analysis was carried out on a Diamonsil C18 (2) reversed-phase column (250 ´ 460 mm, 5 μm) by isocratic elution with acetonitrile and 01% phosphoric acid (50: 50, v/v) The flow rate was 100 ml/min and the detection wavelength was set at 203 nm Linear responses were obtained for polyphyllin I ranging from 2 to 300 µg/ml The intra-and inter-day precisions (RSDs) were less than 923 and 924% with the accuracy (%) ranging from 8765 to 10725% The extraction recovery ranged from 8507 to 9182% with RSD less than 555% Stability studies showed that polyphyllin I and polyphyllin II were stable in preparation and analytical process The results indicated that the validated method was successfully used to determine the concentration-time profiles of polyphyllin I and polyphyllin II Key words: Polyphyllin I, polyphyllin II, pharmacokinetics, reversed phase-high performance liquid chromatography (RP-HPLC), beagle dog plasma