Showing papers by "Jian Wang published in 1999"
TL;DR: The findings showed that p53 gene mutation rate in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan is high and that the mutation types are different from those in UV-induced skin cancers.
Abstract: To understand the role of p53 tumour suppressor gene in the carcinogenesis of arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan, we collected tumour samples from 23 patients with Bowen's disease, seven patients with basal cell carcinomas (BCC) and nine patients with squamous cell carcinomas (SCC). The result showed that p53 gene mutations were found in 39% of cases with Bowen's disease (9/23), 28.6% of cases with BCC (2/7) and 55.6% of cases with SCC (5/9). Most of the mutation sites were located on exon 5 and exon 8. Moreover, the results from direct sequencing indicated that missense mutations were found at codon 149 (C→T) in one case, codon 175 (G→A) in three cases, codon 273 (G→C) in three cases, codon 292 (T→A) in one case, codon 283 (G→T) in one case, codon 172 (T→C) in one case and codon 284 (C→A) in one case. In addition, silent mutations were also found in four cases. These mutations were located at codons 174, 253, 289 and 298 respectively. In immunohistochemistry analysis, p53 overexpression was found in 43.5% (10/23) of cases with Bowen's disease, 14% (1/7) of cases with BCC and 44% (4/9) of cases with SSC. These findings showed that p53 gene mutation rate in arsenic-related skin cancers from the blackfoot disease endemic area of Taiwan is high and that the mutation types are different from those in UV-induced skin cancers. © 1999 Cancer Research Campaign
67 citations
Journal Article•
TL;DR: The characteristics of reduced velocity and increased resistance index in retrobulbar arteries of the AION are demonstrated and CDFI is helpful to the diagnosis of AION.
Abstract: OBJECTIVE To study the characteristics of color Doppler flow imaging (CDFI) in anterior optic neuropathy (AION). METHODS Forty eyes of 25 patients clinically diagnosed to have progressive AION were studied by using CDFI. The peak systolic velocity (PSV), end-diastolic velocity (EDV), time averaged maximum velocity (TAMV) and resistance index (RI) of the central retinal artery (CRA), nasal and temporal posterior ciliary arteries (PCAs) and ophthalmic artery (OA) were detected. The diameter of retrobulbar optic nerve (ON) was detected at the same time. Thirty-four volunteers were served as the control group. RESULTS In the comparison with the control group, there were significantly reduced PSV, EDV and TAMV in PCAs (P < 0.001) and CRA (P < 0.001) and markedly increased RI in PCAs, CRA and OA in AION group. The retrobulbar optic nerve edema was found in 32/40 eyes. CONCLUSIONS The characteristics of reduced velocity and increased resistance index in retrobulbar arteries of the AION are demonstrated. CDFI is helpful to the diagnosis of AION.
1 citations