The invention discloses a method for treating heavy metal contaminated soil. The method comprises the steps of breaking a heavy metal contaminated paddy field, irrigating the paddy field until a water logging layer on a soil layer is 10-15cm deep after breaking the 0.5-0.6m deep soil on the surface layer into soil lumps with sizes less than 2-3mm, adding a modified clay mineral adsorbent, mechanically stirring the adsorbent, standing for 6-8 hours, removing the supernatant, and carrying out solid-liquid separation; then uniformly adding magnetic nano materials to the soil with adjusted moisture content, mechanically stirring the materials, standing for 3-8 hours, and collecting the magnetic nano materials in the soil by way of magnetic separation, thus achieving solid-solid separation; preparing leachate for leaching, adding carbon microspheres while leaching, and then, leaching 1-3 times by using pure water as an eluent, thus obtaining the treated soil, wherein the amount of water used each time is 0.6-1 times the leachate added for the first time by volume. The method is simple to operate, is low in cost, has high removal efficiency, meets the actual requirements, and has good popularization and application values.

Method for treating heavy metal contaminated soil

The invention discloses preparation, application and a usage method for a biocarbon-immobilized composite pollution-degrading bacterium particle. The preparation method comprises: performing thermal cracking on air-dried crushed agricultural waste and animal manure, and grinding and sieving to obtain biocarbon; inoculating a strain of a composite pollution-degrading bacterium into a liquid medium for culturing, after the bacterium strain grows to the logarithmic phase, centrifuging and enriching the degrading bacterium, so as to obtain a composite pollution-degrading bacterium thallus; adding the composite pollution-degrading bacterium thallus into biocarbon, so as to obtain biocarbon adsorbing the composite pollution-degrading bacterium thallus; and adding the biocarbon adsorbing the composite pollution-degrading bacterium thallus into a carrier sol, so as to obtain a composite sol; and dropwise adding the composite sol into a crosslinking liquid to perform low-temperature crosslinking immobilization, and drying to obtain the biocarbon-immobilized composite pollution-degrading bacterium particle. The biocarbon-immobilized composite pollution-degrading bacterium particle has good bioactivity and chemical stability, is capable of effectively removing polycyclic aromatic hydrocarbons in composite polluted soil and accelerating recovery of soil functional microbes, and is low in cost, simple in preparation technology and obvious in restoration effect on polluted soil.

Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle

The invention relates to the technical field of marine environment management, in particular to an offshore oil degrading microbial inoculum and a preparation method thereof. The invention discloses an offshore oil degrading microbial inoculum, which comprises a petroleum degrading flora and a biological fixation carrier, and is characterized in that the microbial inoculum is prepared by attaching the petroleum degrading flora on the floating degradable biological fixation carrier. According to the invention, the biological fixation carrier takes an expanding degradable material as a core, and an outer layer is coated with a degradable composite membrane with positive electricity; the biological fixation carrier has floating property through the expanding degradable material; the degradable composite membrane with positive electricity and a thallus with negative electricity can attract mutually, so that the bacterium are firmly attached on the carrier, and the composite membrane is a porous loose structure, so that the degrading flora can be attached on the wall of the porous structure of the composite membrane; in comparison with the simple petroleum degrading flora, the degrading efficiency of the degrading microbial inoculum is remarkably improved. The offshore oil degrading microbial inoculum, provided by the invention, is expected to be used for bio-remediation of marine oil pollution.

Offshore oil degrading microbial inoculum and preparation method thereof

The invention provides a heavy metal polluted soil repairing agent. The heavy metal polluted soil repairing agent comprises porous carbon and an iron reducing fungicide, and is prepared through the following steps: 1, crushing the porous carbon, and sterilizing the crushed porous carbon; and 2, mixing the iron reducing fungicide with the porous carbon, carrying out shaking culture, adding carboxymethyl cellulose, and uniformly mixing the obtained mixture and carboxymethyl cellulose. The fine structure of the porous carbon and various mineral matters contained in the porous carbon provide a good growing and breeding place for iron reducing bacteria and other active microorganisms; and heavy metals in soil are enriched by using the adsorption effect of the porous carbon, and are passivated by using the iron reducing bacteria adhered on carbon particles, so synergism of the porous carbon and the iron reducing bacteria greatly accelerates the biological repairing process of the heavy metal polluted soil.

Heavy metal polluted soil repairing agent and repairing method thereof

The invention particularly relates to a carbon-based synthetic modified sewage purifier, which is prepared through synthetic modification by taking carbon as a raw material. A preparation method of the sewage purifier is characterized by comprising the steps of: crushing a carbon material, adding water, filtering, baking and sieving to prepare pretreated carbon powder; adding chitosan and sodium alginate into deionized water to prepare a carbon powder modified solution, adding the pretreated carbon powder into the carbon powder modified solution, adding glacial acetic acid, and rolling and forming by using a die to prepare chemically modified carbon powder; activating and cultivating a strain, then centrifuging, and finally diluting with a sterile physiological saline solution to prepare a strain suspension; and mixing and cultivating the chemically modified carbon powder and the strain suspension in proportion, and drying to prepare the carbon-based synthetic modified sewage purifier. The carbon-based synthetic modified sewage purifier has the advantages of rich raw material sources, lower cost, better adsorbability and degradability, no pollution and better application prospect. In addition, the raw materials are sourced from reproducible plants and the product returns to the nature so that a benign closed circulation chain is formed.

Method for preparing carbon-based synthetic modified sewage purifier

The invention relates to a preparation method for magnetic nano biological microspheres for remedying soil polluted by organic chloride durably. The preparation method comprises the following steps of: (1) preparing nano Fe3O4 particles with the surfaces adsorbing amino by a coprecipitation method; (2) dissolving chitosan into an acetic acid solution to form a homogeneous transparent colloidal solution, and mixing and stirring the solution and the nano Fe3O4 particles to obtain the magnetic nano-particles; (3) adding the prepared magnetic nano-particles into a buffering solution of citric acid and performing ultrasonic dispersion to obtain magnetic fluid; and (4) adding the magnetic fluid into a bacterial solution, adding cross-linked fluid dropwise, performing adsorption-cross-linking, and performing solid liquid separation under the action of an external magnetic field to obtain the magnetic nano biological microspheres. The method is quick, simple, convenient and low in cost. The prepared magnetic nano biological microspheres have the characteristics of homogenous shape, large specific surface area, high microbial activity and the like and are applicable to degradation of organic chloride pollutant and in-situ remediation of polluted soil.

Preparation method for magnetic nano biological microspheres for remedying soil polluted by organic chloride

The invention relates to in-situ restoration of pesticide contaminated soil, in particular to a preparation method of microbe immobilized particles for restoring pesticide contaminated soil. Porous carbon is used as a main carrier material, and cellulose, chitosan and sodium alginate are used as auxiliary carriers to realize the purpose of immobilizing the microbes; and the obtained microbe immobilized particles are used for in-situ restoration of the pesticide contaminated soil. The carrier material used in the invention has cheap price, low cost, wide sources, good air permeability and no toxicity to organisms, and can not cause secondary pollution to the soil. The carrier and the immobilizing process thereof can be used for immobilizing multi-bacterium microbes, such as pseudomonas, bacillus, zoogloea, micrococcus and the like, and have certain broad-spectrum applicability; and the bacillus can reach more than 10 billions/gram. Compared with the prior immobilizing method, the invention is characterized by directly sowing the immobilized cell particles into the soil without destroying the original soil structure.

Jinxia Zhang

Papers

Preparation method for magnetic nano biological microspheres for remedying soil polluted by organic chloride

Preparation method of microbe immobilized particles for restoring pesticide contaminated soil

Heavy metal polluted soil repairing agent and repairing method thereof