Abstract Background The range of the ornate dog tick Dermacentor reticulatus is rapidly expanding in Europe. This tick species is the vector of canine babesiosis, caused by Babesia canis , and also plays a role in the transmission of Theileria equi and Babesia caballi in equids. Methods The geographic range of D. reticulatus in the Czech Republic was re-assessed, and an up-to-date distribution map is presented based on material and data obtained during a nationwide citizen science campaign. Received and flagged individuals of D. reticulatus were also analysed for the presence of B. canis DNA. Results In striking contrast to historical records, D. reticulatus was found in all regions of the Czech Republic, with most reports coming from the southeast and northwest of the country. Between February 2018 and June 2021, the project team received 558 photo reports of ticks and 250 packages containing ticks. Of the former, 71.1% were identified as Dermacentor sp. with the remainder identified as Ixodes sp., Haemaphysalis sp., Argas sp. or Hyalomma sp. The majority of specimens in the subset of ticks that were received ( N = 610) were D. reticulatus ( N = 568, 93.7%), followed by Ixodes ricinus and Hyalomma spp. A total of 783 adult D. reticulatus , either received (568) or collected by flagging (215), were tested for the presence of B. canis DNA using species-specific nested PCR targeting part of the 18S rRNA gene; B. canis DNA was demonstrated in 22 samples (2.81%). Conclusions The continuous spread of D. reticulatus in the Czech Republic was documented in this study. In addition, DNA of B. canis was also detected in a number of ticks, suggesting the establishment of B. canis in the Czech Republic. These results suggest that veterinarians need to consider the possibility of canine babesiosis even in dogs without a history of travel. Graphical Abstract 

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The distribution of Dermacentorreticulatus in the Czech Republic re-assessed: citizen science approach to understanding the current distribution of the Babesiacanis vector

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Camera-trapping and capture-recapture models are the most widely used tools for estimating densities of wild felids that have unique coat patterns, such as Eurasian lynx. However, studies dealing with this species are predominantly on a short-term basis and our knowledge of temporal trends and population persistence is still scarce. By using systematic camera-trapping and spatial capture-recapture models, we estimated lynx densities and evaluated density fluctuations, apparent survival, transition rate and individual's turnover during five consecutive seasons at three different sites situated in the Czech–Slovak–Polish borderland at the periphery of the Western Carpathians. Our density estimates vary between 0.26 and 1.85 lynx/100 km2 suitable habitat and represent the lowest and the highest lynx densities reported from the Carpathians. We recorded 1.5–4.1-fold changes in asynchronous fluctuated densities among all study sites and seasons. Furthermore, we detected high individual’s turnover (on average 46.3 ± 8.06% in all independent lynx and 37.6 ± 4.22% in adults) as well as low persistence of adults (only 3 out of 29 individuals detected in all seasons). The overall apparent survival rate was 0.63 ± 0.055 and overall transition rate between sites was 0.03 ± 0.019. Transition rate of males was significantly higher than in females, suggesting male-biased dispersal and female philopatry. Fluctuating densities and high turnover rates, in combination with documented lynx mortality, indicate that the population in our region faces several human-induced mortalities, such as poaching or lynx-vehicle collisions. These factors might restrict population growth and limit the dispersion of lynx to other subsequent areas, thus undermining the favourable conservation status of the Carpathian population. Moreover, our study demonstrates that long-term camera-trapping surveys are needed for evaluation of population trends and for reliable estimates of demographic parameters of wild territorial felids, and can be further used for establishing successful management and conservation measures.

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Multi-seasonal systematic camera-trapping reveals fluctuating densities and high turnover rates of Carpathian lynx on the western edge of its native range

A cat in paradise : hunting and feeding behaviour of Eurasian lynx among abundant naive prey

Dispersal is a key process for the maintenance of intraspecific genetic diversity by ensuring gene flow within and between populations. Despite the ongoing expansion of large carnivores in Europe, lynx populations remain fragmented, isolated, and threatened by inbreeding and loss of genetic diversity. In the course of large carnivore monitoring in the Czech Republic, several biological samples of Eurasian lynx were collected outside the permanent occurrence of this species. Using microsatellite genotyping we identified these as four dispersing lynx males and applied multiple methods (Bayesian clustering in STRUCTURE, Principal Component Analysis (PCA), frequency-based method in GENECLASS2, and machine-learning framework in assignPOP) to assign them to possible source populations. For this we used genotypes from five European lynx populations: the Bohemian-Bavarian-Austrian (N = 36), Carpathian (N = 43), Scandinavian (N = 20), Baltic (N = 15), and Harz (N = 23) population. All four dispersers were successfully assigned to different source populations within Europe and each was recorded at a distance of more than 98 km from the edge of the distribution of the source population identified. Such movements are among the longest described for lynx in Central Europe to this point. The findings indicate the ability of lynx males to disperse in human-dominated landscape thus facilitation of these movements via creation and/or protection of potential migratory corridors together with protection of dispersing individuals should be of high importance in conservation of this iconic predator in Central Europe.

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Long-distance Eurasian lynx dispersal – a prospect for connecting native and reintroduced populations in Central Europe

Abstract In the last decades, large carnivores – the grey wolf (Canis lupus), Eurasian lynx (Lynx lynx) and brown bear (Ursus arctos), and to a certain extent also the wildcat (Felis silvestris) – have increased their distribution ranges throughout Europe. Monitoring of their current distribution and population trends in the Czech Republic is crucial for the effective conservation and elimination of possible conflicts with humans in the future. In the last years, many projects focused on small-scale monitoring of large carnivores were implemented in the Czech Republic and the neighbouring mountain ranges of Slovakia. Using their results, we compiled the dataset from different regions and analysed the recent distribution of large carnivores and the wildcat. The distribution maps are based on verified data on the presence and reproduction in 2012–2016. This is consistent with the standardized methodology used across Europe. The Eurasian lynx was the most widespread of all large carnivore species in the Czech Republic, with the two trans-boundary populations (Carpathian and Bohemian-Bavarian-Austrian) occupying 94 out of 868 squares (10.8%) of the mapping grid of the Czech Republic. Reproduction was confirmed in 46.8% of the occupied squares. The grey wolf occupied 6.8% of the squares in the Czech Republic and its reproduction was confirmed in 10.2% of the occupied squares. Three reproducing packs belonging to the Central European lowland population were confirmed and the area occupied by the species increased three times within the study period. The brown bear occupied 2.8% of the squares of the Czech Republic – the area is restricted to the Carpathians – with no signs of reproduction; its distribution fluctuated heavily during the study period. The wildcat occupied the smallest range of the Czech Republic among the studied species (1.4% of the squares) but its reproduction was confirmed in a trans-boundary area (White Carpathians) at the Slovakian side of the border. The wildcat also significantly increased its range from one to six squares during the study period.

https://content.sciendo.com/downloadpdf/journals/lynx/48/1/article-p93.pdf

Occurrence of large carnivores – Lynx lynx, Canis lupus, and Ursus arctos – and of Felis silvestris in the Czech Republic and western Slovakia in 2012–2016 (Carnivora)

Jiří Beneš

Papers

Occurrence of large carnivores – Lynx lynx, Canis lupus, and Ursus arctos – and of Felis silvestris in the Czech Republic and western Slovakia in 2012–2016 (Carnivora)