Showing papers by "Jitendra P. Khurana published in 1986"
TL;DR: Investigation of the growth and flowering behaviour of a local isolate of S. polyrrhiza, strain SP 20, has revealed that salicylic acid, which is known to have chelating properties itself, induces flowering in this duckweed only in the simultaneous presence of EDTA, in the nutrient solution.
Abstract: Flowering in the genus Spirodela, whether in the laboratory or in nature, has been observed only rarely. In this communication, the growth and flowering behaviour of a local isolate of S. polyrrhiza, strain SP 20 , is being reported. The presence of a chelate, such as EDTA, is obligatory for satisfactory vegetative growth of S. polyrrhiza SP 20 - An optimal flowering response is obtained, however, only when compounds such as EDDHA, a phenolic analog of EDTA, or benzoic acid are supplied. Flowering, so induced, is not influenced by the length of the photoperiod. Flowering fronds become gibbous and both EDDHA and benzoic acid also enhanced anthocyanin content. This investigation has also revealed that salicylic acid, which is known to have chelating properties itself, induces flowering in this duckweed only in the simultaneous presence of EDTA, in the nutrient solution.
11 citations
TL;DR: Flowering in Wolffia microscopica could be induced, under non-inductive long days, by 8-hydroxyquinoline (8-HQ), and was accentuated further when plants, supplied with 8-HQ, were subjected to SD cycles.
Abstract: Wolffia microscopica, a duckweed, flowers in response to a single photoinductive SD cycle of 16 h dark and 8 h light. Flowering in W. microscopica could be induced, under non-inductive long days, by 8-hydroxyquinoline (8-HQ). Flowering was initiated with 10 -6 M 8-HQ and maximum flowering (ca. 75%) was obtained at 5 ×10 -6 M level. Flowering was accentuated further when plants, supplied with 8-HQ, were subjected to SD cycles.
8 citations
TL;DR: Flowering in this strain could be induced even in medium containing normal level of iron, provided the level of EDTA, itself, was raised to 5×10 -4 (5% flowering) or to 10 -3 M (12% flowering), but replacement ofEDTA by EDDHA led to truly remarkable effects.
Abstract: Lemna paucicostata, strain LP 6 , does not ordinarily flower under any photoperiodic schedule, when grown in the modified Bonner-Devirian medium supplemented with 10 -4 M EDTA and 1% sucrose (this is a medium which is otherwise satisfactory for short day induction of flowering of other strains of this duckweed). However, when the ferric citrate concentration in the culture medium containing EDTA was raised 10-fold over that in the normal medium, a low but significant flowering could be initiated in this duckweed, irrespective of the length of the photoperiod. A similar flowering response was obtained when ferric citrate was replaced by ferrous sulphate or ferric chloride, thereby indicating that the inductive effect of higher level of ferric citrate on flowering in strain LP 6 is due to its iron component. Some flowering in this strain could be induced even in medium containing normal level of iron, provided the level of EDTA, itself, was raised to 5×10 -4 (5% flowering) or to 10 -3 M (12% flowering), but replacement of EDTA by EDDHA led to truly remarkable effects. With EDDHA, flowering could be induced at very high levels (90%) under all photoperiodic regimes tried. Floral initiation could be obtained even with 10 -6 M EDDHA, though the optimal level ranged from 5×10 -6 to 10 -5 M.
2 citations