Showing papers by "Johannes Huebner published in 1993"
TL;DR: The study suggests that colonisation withP.
Abstract: Objective
A 4 month prospective study was performed to assess the incidence and routes of endogenous or exogenous colonization and nosocomial infection caused byStaphylococcus aureus andPseudomonas aeruginosa in surgical critically ill patients.
65 citations
TL;DR: In this paper, ribosomal DNA restriction fragment length polymorphism analysis (ribotyping) was applied to analyze Pseudomonas aeruginosa isolated during a 4-month prospective study.
Abstract: To elucidate the sources of Pseudomonas aeruginosa on a surgical intensive care unit, rDNA restriction fragment length polymorphism analysis (ribotyping) was applied to analyze strains isolated during a 4-month prospective study. Samples included 1635 from 153 patients, 2463 from 97 staff members, and 581 from the environment. Only 18 patients were colonized. Isolation from their animate and inanimate environment was very low, with 3 and 2 samples, respectively, being positive. Samples from tap water were negative. Ribotyping could easily distinguish 16 different digest patterns with identical follow-up isolates of the same patient. Horizontal transmission occurred only twice. The discriminatory power of ribosomal DNA in differentiating strains was dependent on the restriction enzymes used; among eight different enzymes, PvuII was the most sensitive, producing 15 different patterns. Ribotyping showed high sensitivity in typing P. aeruginosa isolates and confirmed that colonization occurs from endogenous rather than from exogenous sources.
54 citations
TL;DR: A case of Shigella dysenteriae bacteremia in a 39-year-old HIV-positive patient is reported, although the patient presented with bloody and watery diarrhea, stool cultures failed to grow enteric pathogens.
Abstract: Shigella bacteremia in adult patients is rare and is usually associated with immunosuppressive diseases. To date, 12 HIV-positive patients withShigella bacteremia have been reported in the medical literature. We report a case ofShigella dysenteriae bacteremia in a 39-year-old HIV-positive patient. Although the patient presented with bloody and watery diarrhea, stool cultures failed to grow enteric pathogens. The patient responded well to appropriate antibiotic therapy.
26 citations
TL;DR: In an in vitro model, the activity of sodium bisulfite, heparin with chlorbutol or chlorcresol as preservatives, and their combination with cefpirom for treating the internal surface of polyurethane and silicone catheters colonized with Staphylococcus aureus and Staphyllococcus epidermidis was studied.
Abstract: Device-related infections often persist until the device is removed because systemic antibiotic therapy fails frequently In an in vitro model, the activity of sodium bisulfite (an antioxidant added to many drugs) heparin with chlorbutol or chlorcresol as preservatives, and their combination with cefpirom for treating the internal surface of polyurethane and silicone catheters colonized with Staphylococcus aureus and Staphylococcus epidermidis was studied Sodium bisulfite (005%) markedly reduced catheter colonization Heparin combined with chlorbutol, 1000 micrograms/mL of cefpirom, and 005% of sodium bisulfite was not more effective than sodium bisulfite alone A lesser effect was shown by treating adherent bacteria on both catheters with heparin plus preservatives alone Not only antibiotics but also other substances with antibacterial activity should be evaluated for treatment and prophylaxis of catheter-related infection
16 citations