J
John F. Reidhaar-Olson
Researcher at Massachusetts Institute of Technology
Publications - 4
Citations - 2963
John F. Reidhaar-Olson is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Protein structure & Site-directed mutagenesis. The author has an hindex of 4, co-authored 4 publications receiving 2956 citations.
Papers
More filters
Journal ArticleDOI
Deciphering the message in protein sequences: tolerance to amino acid substitutions
TL;DR: Comparison of different sequences with similar messages can reveal key features of the code and improve understanding of how a protein folds and how it performs its function.
Journal ArticleDOI
Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences
TL;DR: Repeated application of this method to the dimer interface of the DNA-binding domain of lambda repressor reveals that the number and type of substitutions allowed at each position are extremely variable.
Book ChapterDOI
Random mutagenesis of protein sequences using oligonucleotide cassettes.
John F. Reidhaar-Olson,James U. Bowie,Richard M. Breyer,James C. Hu,Kendall L. Knight,Wendell A. Lim,Michael C. Mossing,Dawn A. Parsell,Kevin R. Shoemaker,Robert T. Sauer +9 more
Journal ArticleDOI
An essential proline in lambda repressor is required for resistance to intracellular proteolysis.
TL;DR: Double-mutant studies show that this instability contributes to but is not the main cause of its rapid intracellular degradation and also suggest that proteolysis proceeds from the denatured forms of proteins containing the PA78 substitution.