J
Johnston Sd
Publications - 3
Citations - 80
Johnston Sd is an academic researcher. The author has contributed to research in topics: Whole blood & Luteal phase. The author has an hindex of 3, co-authored 3 publications receiving 80 citations.
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Effect of time and temperature on bovine serum and plasma progesterone concentration
TL;DR: The incubation time and temperature of whole blood, from collection of blood to the separation of serum or plasma, significantly affects assayable concentration of progesterone.
Journal Article
Decline in assayable progesterone in bovine plasma: effect of time, temperature, anticoagulant, and presence of blood cells.
TL;DR: Jugular vein blood samples collected from 5 Holstein-Friesian cows during the luteal phase of the estrous cycle were incubated at 4 C, 22 C, and 37 C for 0, 0.5, 1, 2, 4, 6, 8, 12, and 24 hours, and plasma was frozen for later progesterone assay.
Journal Article
Role of blood cells in degradation of progesterone in bovine blood.
TL;DR: Potassium oxalate-sodium fluoride anticoagulant and refrigeration provided the best progesterone results when blood samples were not processed immediately after collection, and degradation of bovine blood progestersone by blood cells was dependent on the glycolytic process.