https://estudogeral.sib.uc.pt/bitstream/10316/3921/1/filedf0c1cd6e7c84346873618a4bbc44d6a.pdf

Plant community tolerant to trace elements growing on the degraded soils of São Domingos mine in the south east of Portugal: environmental implications.

Metal speciation in solid matrices

Analysis of serpentinophytes from north-east of Portugal for trace metal accumulation--relevance to the management of mine environment.

An analysis of the molecular diversity of N(2) fixers and denitrifiers associated with mangrove roots was performed using terminal restriction length polymorphism (T-RFLP) of nifH (N(2) fixation) and nirS and nirK (denitrification), and the compositions and structures of these communities among three sites were compared. The number of operational taxonomic units (OTU) for nifH was higher than that for nirK or nirS at all three sites. Site 3, which had the highest organic matter and sand content in the rhizosphere sediment, as well as the lowest pore water oxygen concentration, had the highest nifH diversity. Principal component analysis of biogeochemical parameters identified soil texture, organic matter content, pore water oxygen concentration, and salinity as the main variables that differentiated the sites. Nonmetric multidimensional scaling (MDS) analyses of the T-RFLP data using the Bray-Curtis coefficient, group analyses, and pairwise comparisons between the sites clearly separated the OTU of site 3 from those of sites 1 and 2. For nirS, there were statistically significant differences in the composition of OTU among the sites, but the variability was less than for nifH. OTU defined on the basis of nirK were highly similar, and the three sites were not clearly separated on the basis of these sequences. The phylogenetic trees of nifH, nirK, and nirS showed that most of the cloned sequences were more similar to sequences from the rhizosphere isolates than to those from known strains or from other environments.

/pdf/molecular-characterization-of-diazotrophic-and-denitrifying-hmoovap7hz.pdf

Molecular Characterization of Diazotrophic and Denitrifying Bacteria Associated with Mangrove Roots

The different aspects of speciation analysis are reviewed. Species-specific instrumental techniques as well as various speciation schemes are considered for the determination of species of metals and metalloids, including organometallic compounds. The application of the methods are discussed in some detail for the analysis of natural waters, air, soil, sediment and biological samples. The relationship between metal species and bioavailability is also briefly dealt with.

Speciation analysis — why and how?

Techniques and Instrumentation Basic Materials Sample Decomposition Botanical and Zoological Samples Food Samples Clinical Samples Water Samples Air Samples Soils and Related Samples Determination of Metal Species Some Important Developing Areas in Trace Metal Analysis for Biological and Environmental Samples.

http://library.um.ac.id/free-contents/downloadpdf.php/buku/selected-methods-of-trace-metal-analysis-biological-and-environmental-samples-jon-c-van-loon-12344.pdf

Jon C. Van Loon

Papers

Selected methods of trace metal analysis : biological and environmental samples / Jon C. Van Loon