抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

THE DESIGN AND ANALYSIS OF EXPERIMENTS. By Oscar Kempthorne. New York, John Wiley and Sons, Inc., 1952. 631 pp. $8.50. This book by a teacher of statistics (as well as a consultant for \"experimenters\") is a comprehensive study of the philosophical background for the statistical design of experiment. It is necessary to have some facility with algebraic notation and manipulation to be able to use the volume intelligently. The problems are presented from the theoretical point of view, without such practical examples as would be helpful for those not acquainted with mathematics. The mathematical justification for the techniques is given. As a somewhat advanced treatment of the design and analysis of experiments, this volume will be interesting and helpful for many who approach statistics theoretically as well as practically. With emphasis on the \"why,\" and with description given broadly, the author relates the subject matter to the general theory of statistics and to the general problem of experimental inference. MARGARET J. ROBERTSON

The Design and Analysis of Experiments

Membranes have an increasingly important role in alleviating water scarcity and the pollution of aquatic environments. Promising molecular-level design approaches are reviewed for membrane materials, focusing on how these materials address the urgent requirements of water treatment applications.

Materials for next-generation desalination and water purification membranes

The rapid rise of shale gas development through horizontal drilling and high volume hydraulic fracturing has expanded the extraction of hydrocarbon resources in the U.S. The rise of shale gas development has triggered an intense public debate regarding the potential environmental and human health effects from hydraulic fracturing. This paper provides a critical review of the potential risks that shale gas operations pose to water resources, with an emphasis on case studies mostly from the U.S. Four potential risks for water resources are identified: (1) the contamination of shallow aquifers with fugitive hydrocarbon gases (i.e., stray gas contamination), which can also potentially lead to the salinization of shallow groundwater through leaking natural gas wells and subsurface flow; (2) the contamination of surface water and shallow groundwater from spills, leaks, and/or the disposal of inadequately treated shale gas wastewater; (3) the accumulation of toxic and radioactive elements in soil or stream sediments near disposal or spill sites; and (4) the overextraction of water resources for high-volume hydraulic fracturing that could induce water shortages or conflicts with other water users, particularly in water-scarce areas. Analysis of published data (through January 2014) reveals evidence for stray gas contamination, surface water impacts in areas of intensive shale gas development, and the accumulation of radium isotopes in some disposal and spill sites. The direct contamination of shallow groundwater from hydraulic fracturing fluids and deep formation waters by hydraulic fracturing itself, however, remains controversial.

/pdf/a-critical-review-of-the-risks-to-water-resources-from-2v7n5hmzlz.pdf

A Critical Review of the Risks to Water Resources from Unconventional Shale Gas Development and Hydraulic Fracturing in the United States

Annual Energy Outlook

Unconventional natural gas resources offer an opportunity to access a relatively clean fossil fuel that could potentially lead to energy independence for some countries. Horizontal drilling and hydraulic fracturing make the extraction of tightly bound natural gas from shale formations economically feasible. These technologies are not free from environmental risks, however, especially those related to regional water quality, such as gas migration, contaminant transport through induced and natural fractures, wastewater discharge, and accidental spills. We review the current understanding of environmental issues associated with unconventional gas extraction. Improved understanding of the fate and transport of contaminants of concern and increased long-term monitoring and data dissemination will help manage these water-quality risks today and in the future.

https://science.sciencemag.org/content/sci/340/6134/1235009.full.pdf

Impact of shale gas development on regional water quality

State highway agencies in Connecticut, Kansas, Minnesota, Wisconsin, and Wyoming have successfully designed and constructed rigid pavements containing recycled concrete aggregate (RCA). Success has been attributed in part to the minimization of old mortar content in the RCA during recycling processes, thereby controlling the total mortar content of the new portland cement concrete (PCC) mixture, or to the achievement of higher-than-expected compressive strengths through adjustments in mix proportions, or both. There was no clear correlation between mortar content and cracking distresses in field investigations, although one project did exhibit significantly more slab cracking in the recycled pavement than in the corresponding control pavement. The increased cracking may have been due to the large differences in total mortar content between the recycled and control sections. In general, the recycled PCC pavements considered in this study have performed comparably with their conventional PCC pavement counterparts, including the recycled pavements that incorporated RCA derived from concrete affected by D-cracking and alkali-silica reactivity (ASR). There is, however, evidence of small amounts of localized recurrent ASR in the recycled Wyoming pavement. Whether this reactivity will eventually develop into widespread distress remains to be seen.

Performance of Rigid Pavements Containing Recycled Concrete Aggregates

Thin and ultrathin whitetopping overlays are becoming a more common method of pavement rehabilitation. It is important to gain information on the types of distresses that occur in the overlays and effective repair techniques. In 1997 the Minnesota Department of Transportation constructed several thin and ultrathin whitetopping test cells at the Minnesota Road Research (Mn/ROAD) facility. Typical distresses included corner breaks, transverse cracks, and reflective cracks. The finite element program ISLAB2000 was used to investigate stress patterns and their relation to the distresses. Different techniques for repairing ultrathin whitetopping were investigated. Various techniques were also used to deter reflective cracking, including various bond-breaking materials and full-depth sawing at strategic locations along the longitudinal joint to prevent cracks from propagating into adjacent panels at misaligned transverse joints. Four of the six sections had present serviceability indexes (PSIs) greater than 3.5...

Performance, Analysis, and Repair of Ultrathin and Thin Whitetopping at Minnesota Road Research Facility:

Gases can migrate into the cemented annulus of a wellbore during early gelation when hydrostatic pressure within the cement slurry drops. Different means to describe hydrostatic-pressure reduction have been proposed and reported in the literature. Among them, static gel strength (SGS) is the most widely accepted concept in describing the strength development of hydrating cement. The classic shear-stress theory uses SGS to quantify the hydrostaticpressure reduction in the cement column. Approaches derived from the concept of SGS have contributed to understanding mechanisms of gas migration and methods of minimizing it. Unfortunately, these approaches do not accurately predict gas migration. Although SGS was originally adopted to describe the shear stress at interfaces, it has also been used to estimate the shear resistance required to deform slurry during the hydration period. Before early gelation, the hydrostatic pressure will overcome the formation gas pressure and prevent gas migrations. During gelation, the cement develops enough rigidity to withstand the gas invasion. This critical hydration period is defined as the transition time. API STD 65-2 (API 2010a) provides standards for determining the transition time by use of the concept of SGS. Current industry practice is to reduce the transition time, thereby lowering the potential for invading gas introducing migration pathways in the cemented annulus. This approach, although certainly helpful in reducing the risk for gas migration, does not eliminate its occurrence. Experimental results presented in this study demonstrate that the relationship between SGS and hydrostatic-pressure reduction is not linear. Characteristics of the transition-time endpoints depend on slurry properties and downhole conditions. Moreover, SGS is not able to characterize the gas-tight property of a cement slurry. When slurry gels, the mechanical properties are governed by its growing solid fraction. The gel can deform under shear loading, but gases and other fluids will need to break or fracture the bond between solids and push them aside for pathways to form within the cement/matrix domain at this point. To fully understand this process, the bond strength between solid particles and the compressibility of the cement matrix are needed. The bond strength and compressibility are mechanical properties dependent on the changing rigidity of the gelling cement. However, SGS does not address these important properties and, therefore, SGS is limited in its ability to predict gas-migration potential. A better means to characterize the cement/matrix strength by use of fundamental concepts and variables for replacing SGS is desired.

Theory-Based Review of Limitations With Static Gel Strength in Cement/Matrix Characterization

In this article, we describe the capability of a nondestructive evaluation (NDE) method at determining the strength of concrete surfaces. The method uses highly non-linear solitary waves (HNSWs) propagating inside a metamaterial in contact with the concrete to be tested. The metamaterial consists of a chain of spherical particles, and is part of a built-in transducer designed to excite and detect HNSWs. The NDE method exploits the dynamic interaction between the metamaterial and the concrete, and the hypothesis is that this interaction depends on the stiffness of the specimen being inspected. In this study, we tested this hypothesis by assembling two kinds of transducer and by casting cylinders with different water-to-cement ratios. The results show that the time of flight of the HNSWs is inversely proportional to the modulus of elasticity of the concrete. Once fully developed, the proposed NDE method may easily assess concrete surfaces.

Julie M. Vandenbossche

Papers

Impact of shale gas development on regional water quality

Performance of Rigid Pavements Containing Recycled Concrete Aggregates

Performance, Analysis, and Repair of Ultrathin and Thin Whitetopping at Minnesota Road Research Facility:

Theory-Based Review of Limitations With Static Gel Strength in Cement/Matrix Characterization

Nondestructive testing of concrete using highly nonlinear solitary waves