Jun Zhang

TL;DR: A highly automated bioanalytical platform featuring MS/MS method optimization by automated infusion, with a customized in-house software tool in support of a Metstab screen, which has achieved and maintained a monthly throughput of 1300 compound assays representing 500 discrete compounds per instrument per month on this platform.

TL;DR: Recent advances such as automated MS/MS optimization, high-speed and multiplexed LC separation, and integrated software support have significantly increased the speed and quality of ADMET bioanalysis using LC-MS/MS.

TL;DR: Various recent developments that facilitate automation of individual bioanalytical procedures, such as sample preparation, MS/MS method development, sample analysis and data review, as well as fully integrated software tools that manage the entireBioanalytical workflow in HT discovery bioanalysis are reviewed.

TL;DR: To improve overall efficiency of ADME bioanalysis, an integrated system was proposed featuring an automated and unattended MS/MS optimization, a staggered parallel LC/MS/MS for high-throughput sample analysis, and a sophisticated software tool for liquid chromatography/tandem mass spectrometry raw data review as well as biological data calculation and reporting.

TL;DR: Cassette incubation with bioanalysis using HRMS is a feasible approach for high-throughput in vitro ADME assays evaluated in this study.