Lehrbuch und atlas des spaltlampenmikroscopie des lebenden Augee

https://acsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/1097-0142%28195809/10%2911%3A5%3C873%3A%3AAID-CNCR2820110503%3E3.0.CO%3B2-B

A new and rapid method for diagnosis of vaginal and cervical cancer by fluorescence microscopy.

VI. Summary Qualitative analysis for the intracellular localization of nucleic acids may be performed with reasonable assurance by staining techniques, and confirmed by the use of specific nucleolytic enzymes on fixed tissues. Ultraviolet microscopy serves similarly, but can contribute little, if any, further information in this sphere. The newer methods of X-ray absorption, infrared microscopy, etc., at present find greater application in aspects of histochemistry other than nucleic acid analysis. Quantitative analysis is beset with errors because of uncertainties about specificity, stoichiometry, diffusion, and optical factors. Reproducibility has been demonstrated for a number of quantitative procedures. Although essential, it is not in itself an adequate test of the reliability of the method. Thus, repeated analysis of nuclei of the same tissue, fixed in the same manner, hydrolyzed at constant temperature and constant time, and otherwise treated identically in the Feulgen procedure may be expected to give reproducible absorption values. Nevertheless, one may not conclude that extension of the procedure to other nuclei will give results which may be validly compared (see discussion of effect of heterogeneity above). It is, therefore, necessary to evaluate the multitude of quantitative data in the literature with an awareness of the possible errors. It is our opinion that for a procedure to qualify as a quantitative method it must be possible to state the results in absolute values so that they may be tested (in suitable material) by independent methods (such as macrochemical analysis). This has been done for ultraviolet analysis of nucleic acid in whole thymus nuclei (Kurnick and Thorell in Kurnick, 1950b; Leuchtenberger et al., 1952). We have also carried out such studies for methyl green staining in a number of tissues. However a wider variety of tissues should probably be studied before the method is extended to tissues in which confirmation of the result by chemical means is not feasible. The potential error in the assumptions as to uniformity in degree of polymerization and absence of competing substances (as proteins bound to phosphoric acid groups and not extracted by dilute HCl) must be borne in mind in attempting to extend the observations on ultraviolet absorption and methyl green staining. It is hoped that independent tests of other quantitative histochemical methods will be reported to assist in the evaluation of a large and increasing bulk of quantitative histochemical analyses by methods not yet adequately examined. Until this has been done, histochemical analyses for nucleic acids should be regarded as primarily qualitative, and interpretations based upon quantitation must be viewed with caution.

Histochemistry of Nucleic Acids

For complete spherical concentration of light the maximum theoretically possible total energy density for a given power input can be, in principle, achieved by appropriate choice of polarization and angular amplitude variation. Illumination of a focusing system with a plane-polarized wave creates at the focus equal electric and magnetic energy densities. By appropriate choice of radial variation this energy density can be maximized. For hemispherical concentration the electric energy density can be seven sixteenths of the maximum possible for a given power input, and the total energy density can be seven eighths of the maximum possible. Focusing by optical systems satisfying the sine condition amongst others is also considered. For a system satisfying the sine condition, the total energy density can be 64/75 of the maximum possible.

http://nontrivialzeros.net/KGL_Papers/13_OptimalConcentration_JMO_1994.pdf

Optimal Concentration of Electromagnetic Radiation

The resolution of lenses is normally limited by the wave nature of light. Imaging with perfect resolution was believed to rely on negative refraction, but here we present experimental evidence for subwavelength imaging with positive refraction.

/pdf/evidence-for-subwavelength-imaging-with-positive-refraction-53zefmk3rp.pdf

Evidence for subwavelength imaging with positive refraction

Die vorliegende Arbeit enthalt die Ergebnisse photoelektrischer Absorptionsmessungen bei der serosen Entzundung, die experimentell durch Dichlordiathylsulfid hervorgerufen wurde. Die Befunde stimmen im wesentlichen mit den Ergebnissen der vorangegangenen Untersuchungen uber das gleiche Thema uberein; lediglich der Einflus der Formolfixierung scheint doch groser zu sein als zunachst vermutet. Es zeigt sich, das das entzundliche Odem die gleichen Extinktionswerte hat wie die Blutflussigkeit. Dennoch durften es bestimmte, von einer primaren Zellschadigung ausgehende Wirkstoffe sein, wie sie in der fruheren Arbeit nachgewiesen wurden, die dem entzundlichen Odem seine eigentlich charakteristischen Eigenschaften verleihen.

Über den Ablauf der serösen Entzündung

Über den Zellstoffwechsel in der Milz nach Sensibilisierung

Mathematical Theory of Optics. R. K. Luneburg. With a foreword by Emile Wolf and supplementary notes by M. Herzberger. University of California Press, Berkeley, 1964. xxx + 448 pp. Illus. $12.50

Jürgen Meyer-Arendt

Papers

Über den Ablauf der serösen Entzündung

Über den Zellstoffwechsel in der Milz nach Sensibilisierung

Mathematical Theory of Optics. R. K. Luneburg. With a foreword by Emile Wolf and supplementary notes by M. Herzberger. University of California Press, Berkeley, 1964. xxx + 448 pp. Illus. $12.50

Mikromethode der elektrophoretischen Eiweißtrennung

Zur Perchlorsäureextraktion von Ribosenucleotiden