Showing papers by "Karen W. Hughes published in 1989"
TL;DR: In another study, the authors showed that growing tetraploid tobacco plants from cell lines derived from cultured cell lines (TC) can increase the yield of F1 plants from 2% to as much as 25% indicating that embryo rescue is possible.
Abstract: Tetraploid tobacco plants (Nicotiana tabacum) derived from cultured cell lines (TC) are partially cross-incompatible with their diploid progenitors (C). C x TC crosses (TC-derived tetraploids as the pollen parent) yield only 2% viable seed. The remaining seeds are normal size but lack an embryo (apoembryonic seeds). Apoembryonic seeds do not occur in the reciprocal TC x C crosses. Sections of ovules from C x TC crosses revealed that an embryo formed but that embryo growth slowed at 5 days postpollination and that by the 12th day following pollination, the embryo had disappeared although the ovule continued to develop. Endosperm degeneration occurred concurrently with embryo death. Culturing ovules from C x TC crosses has increased the yield of F1 plants from 2% to as much as 25% indicating that embryo rescue is possible. Surviving F1 plants from TC x C crosses have close to triploid chromosome numbers and are fertile. WHEN PLANT TISSUES are placed in culture, there is sometimes an increase in point mutations and chromosomal aberrations (Scowcroft and Larkin, 1982). In previous studies, tobacco plants (Nicotiana tabacum var. Wis. 38) were regenerated from both herbicide (paraquat)resistant and wild type cell lines (Miller and
11 citations