Katherine Gordon

TL;DR: In this paper, the authors describe a sequence containing a gene encoding a protein, the gene being under the transcriptional control in the DNA sequence of a mammalian milk protein promoter which does not naturally control the transcription of the gene, such DNA sequence including DNA enabling secretion of the protein.

TL;DR: The first successful production of transgenic goats that express a heterologous protein in their milk was reported in this article, where the first animal, a female, was mated and allowed to carry the pregnancy to term.

TL;DR: In this article, an exogenous gene was expressed in the mammary epithelium of transgenic mice in the hope that the encoded protein would be secreted into milk, and the protein was found to be biologically active.

TL;DR: It is demonstrated that the WAP gene promoter can target the expression of a transgene to the mammary gland and that this expression is inducible during lactation.

TL;DR: Analysis of the transgenic protein indicated that it had a significantly different carbohydrate composition from the recombinant enzyme produced in mouse C127 cells, and molecular size analysis of the oligosaccharides confirmed their differences and showed that the mouse cell-derived preparation contained larger, complex-type N-linked oligOSaccharide structures than the material produced in goat mammary tissue.