Showing papers by "Kenji Matsumoto published in 1999"

Functional expression of transmembrane 4 superfamily molecules on human eosinophils.

Abstract: Background: Transmembrane 4 superfamily (TM4SF) molecules are exclusively found on hematopoietic cells. Several members of the TM4SF are reported to be associated with other cell surface molecules, including integrins, and might participate in signal transduction, but little is known about their role on eosinophils. In the present study, we determined the expression and function of TM4SF molecules on human eosinophils. Methods: Surface expression of TM4SF molecules on purified peripheral blood eosinophils was examined using indirect immunofluorescence and flow cytometry. Purified eosinophils were incubated with anti-TM4SF monoclonal antibodies (mAbs) for up to 24 h. Eosinophil activation was evaluated by measuring eosinophil homotypic aggregation as well as changes in surface expression of CD11b or CD62L by flow cytometry. Results: Freshly isolated eosinophils expressed CD9, CD37, CD53, CD63 and CD81. Incubation with anti-CD9 mAb but not with anti-CD37, CD53, CD63 or CD81 mAb induced significant eosinophil homotypic aggregation. Incubation with any of the anti-TM4SF mAb for 30 min failed to alter the expression of either CD11b or CD62L on eosinophils. In contrast, the expression of CD11b was significantly enhanced after 24 h of incubation with anti-CD53 mAb, while the expression of CD62L was significantly reduced with anti-CD81 mAb. Conclusions: Cross-linking of some surface TM4SF molecules induced significant eosinophil homotypic aggregation, upregulation of CD11b expression, or CD62L shedding, consistent with activation of eosinophils. Our data suggest that several TM4SF molecules are functionally expressed on human eosinophils, and therefore might participate in allergic inflammation.

Image forming method and device

Abstract: PROBLEM TO BE SOLVED: To provide a digital copying machine by which an original larger than a maximum placing size of an original platen board is copied at a low cost in a processing time of a practical level. SOLUTION: Image data of devided originals outputted from an original scanning input means 1 are converted into reduced image data in response to a copy size by an image reduction means 2, the reduced image data are stored in a data storage means 3, and a pattern matching means 4 compares common image data in the reduced divided image data to discriminate the coincidence of the image data. An address setting means 5 generates a joint position address corresponding to each joint position in each storage area based on the discrimination result. A data output control means 6 joints sequentially the reduced image data of adjacent images stored in each storage area and reads them. An image output means 7 generates a copied image of the original onto a prescribed recording medium according to the reduced image data.