The present invention relates to a functional health food for preventing or ameliorating arthritis containing a DNA fragment mixture consisting of a DNA fragment mixture with molecular weight of 2500-5000 kDa inclusive, a DNA fragment mixture with molecular weight greater than or equal to 50 and less than 2500 kDa, and a DNA fragment mixture with molecular weight greater than or equal to 5 and less than 50 kDa. The DNA fragment mixture significantly suppresses production of inflammatory cytokines known as prevention indicators for degenerative arthritis when orally administered. Owing to excellent and long-lasting fast-acting properties and consistency, the DNA fragment mixture can be useful as functional health foods for preventing or ameliorating arthritis.

DNA Health functional food comprising mixture of DNA fragments for preventing or improving osteoarthritis

The present specification relates to polymer nanoparticles in which nucleic acid fragments are encapsulated and a method for manufacturing the same. The polymer can be a mucoadhesive polymer, specifically chitosan, and the nucleic acid fragment can be PDRN. The polymer nanoparticle in which the nucleic acid fragments are encapsulated can be used as eye drops. The polymer nanoparticles in which the nucleic acid fragments are encapsulated can be combined with intraocular mucosa, thereby being able to exhibit an effect of slowly releasing nucleic acid fragments in the intraocular mucosa. In addition, the polymer nanoparticles in which the nucleic acid fragments are encapsulated can exhibit high efficiency at a low drug dosage in eyes, thereby exhibiting convenience or cost reduction effects for a patient.

PDRN Eye drops containing PDRN encapsulated chitosan nanoparticles and method for manufacturing the same

The present invention relates to manufacture of a high purity DNA fragment mixture. Provided is a method for manufacturing a high purity DNA fragment mixture comprising: (a) a step of preparing a testicle crushed liquid by thawing a fish testicle, removing blood and foreign materials, adding purified water to the thawed testicle, and crushing the same; (b) a step of decomposing testicle cells and reducing the molecular weight thereof by inputting a sodium chloride solution and a sodium lauryl sulfate solution to the testicle crushed liquid of the step (a), maintaining a reaction temperature at 90-100°C, and stirring the same for 0.5 to 10 hours; and (c) a step of purification by cooling the reaction liquid to room temperature after the step (b), treating the same with activated carbon, and removing protein and fat.

DNA Manufacturing method of high purity DNA fragments mixtures

The present invention relates to a composition, which is for repairing rotator cuff tears and includes nucleic acid and chitosan. More specifically, a composition for repairing rotator cuff tears and prepared by mixing nucleic acid and chitosan was ascertained to undergo a temperature-dependent sol-gel transition and have a greater tissue-repairing effect than typical individual compositions, and it was ascertained that the rate of decomposition of the composition was slowed and the residence time of the composition in a tear region was increased so that the tissue-repairing effect was prolonged. Thus, it is expected that by using the composition of the present invention, which is for repairing rotator cuff tears and includes nucleic acid and chitosan, it will be possible to develop a therapeutic composition which is remarkably effective for repairing torn rotator cuff tissue.

Composition comprising nucleic acid and chitosan for repair of rotator cuff tear

According to an embodiment of the present invention, a battery charger of fishing goods is composed by including a mother battery and a plurality of first and second terminal clips According to an embodiment of the present invention, the battery charger of fishing goods charges the mother battery by using a commercial power, mounts a battery for fishing goods on the first and second terminal clips, charges by using a charging power of the mother battery, prevents overcharging of the battery by having an overcharging prevention circuit and can store the battery without being removed even when the charging of the battery is completed

Battery charger of fishing goods

PURPOSE: A composition for cartilage regeneration including a DNA fragment mixture separated from fish testis is provided to increase lubrication and buffering effects of cartilage when injected in a joint due to high viscosity by including a DNA fragment mixture with a molecular volume in a specific range. CONSTITUTION: A composition for cartilage regeneration comprises a DNA fragment mixture with a molecular weight of 3,000-10,000 kDa separated from fish testis. A fish is a fish of a salmon family. A manufacturing method of the composition for cartilage regeneration comprises a step of obtaining fraction liquid of the DNA fragment mixture with a molecular weight of 3,000-10,000 kDa from a gel filtration filter liquor; a step of precipitating the DNA fragment mixture by stirring after adding isopropyl alcohol or ethanol to filter liquor; and a step of dehydrating and drying the DNA fragment mixture after washing the same with an ethanol water solution or isopropyl alcohol. [Reference numerals] (AA) (First process) Step of defrosting fish testis and removing blood and foreign materials; (BB) (Second process) Step of putting refined water into the defrosted fish testis, pulverizing, and manufacturing testis pulverized liquid; (CC) (Third process) Step of mixing the testis pulverized liquid of the second process and a sodium chloride aqueous solution, reacting while stirring, and cooling; (DD) (Fourth process) Step of adding a sodium hydroxide aqueous solution into the cooled reactant liquid of the third process, adjusting into pH 10-12, stirring, filtering, and obtaining filtered liquid; (EE) (Fifth process) Step of adding hydrochloric acid into the filtered liquid of the fourth process to be pH 6.5-8.5, reacting, cooling, filtering, and obtaining filtered liquid containing a DNA fragment mixture; (FF) (Sixth process) Step of gel filtering the filtered liquid of the fifth process and obtaining fraction liquid of the DNA fragment mixture of 3,000-10,000kDA molecular weight; (GG) (Seventh process) Step of adding isopropyl alcohol or ethanol into the filtered liquid of the sixth process, stirring, and precipitating a DNA fragment mixture; (HH) (Eighth process) Step of washing the DNA fragment mixture of the seventh process with an ethanol aqueous solution or isopropyl alcohol, dehydrating, and drying

Composition comprising mixture of dna fragments separated from fish's testis for regenerating cartilage

The present invention relates to a viscous eye crop composition comprising polydeoxyribonucleotide and a method for manufacturing the same which increases the viscosity by adding a cationic polymer. The viscous eye drop composition according to the present invention is safe in vivo by using polydeoxyribonucleotide which is a component of a living body, can properly maintain isotonicity, when used as an eye drop, since an osmotic pressure does not overly increase, and is favorable for corneal penetration. Further, effectiveness of a drug can be maximized by improving weaknesses in pharmaceutical retentivity of conventional eye drops due to low viscosity.

Thickened eye drop composition comprising polydeoxyribonucleotide and method thereof

The present invention relates to a pharmaceutical composition for preventing or treating rotator cuff rupture comprising a mixture of DNA fragments separated from fish′s semen or testis an active ingredients. It has effects on increasing rate of wound healing and promoting functional recovery as well as decreasing pains by injecting the pharmaceutical composition of the present invention into a lesion diagnosed with rotator cuff rupture.

Pharmaceutical composition comprising mixture of dna fragments separated from fish's semen or testis for the prevention or treatment of rotator cuff tear

The present invention relates to composition comprising mixture of DNA fragments separated from fishs semen or testis for prevention or treatment of ischemic colitis which is confirmed to have excellent prevention or treatment effects on the ischemic colitis. Also, the composition for prevention or treatment of the ischemic colitis in the present invention is safe even during a long-term administration and has little side effects. Accordingly, the composition is expected to be helpful for the treatment of the ischemic colitis by developing outstanding remedy of the ischemic colitis which is safe, has little side effects and has excellent treatment effects by using the composition for prevention or treatment of the ischemic colitis.

DNA Composition comprising mixture of DNA fragments separated from fish's semen or testis for the prevention or treatment of ischemic colitis

The present invention relates to an adhesion prevention agent comprising a biocompatible natural polymer and a DNA fragment mixture, and relates to a manufacturing method thereof. The adhesion prevention agent of the present invention has excellent effects in preventing the adhesion by exhibiting excellent biocompatibility. The adhesion prevention agent has effects in increasing a healing speed of a damaged region of the tissue due to the surgery, by reducing an inflammatory reaction and increasing the healing speed.

Kim Ik Soo

Papers

Composition comprising mixture of dna fragments separated from fish's testis for regenerating cartilage

Thickened eye drop composition comprising polydeoxyribonucleotide and method thereof

Pharmaceutical composition comprising mixture of dna fragments separated from fish's semen or testis for the prevention or treatment of rotator cuff tear

DNA Composition comprising mixture of DNA fragments separated from fish's semen or testis for the prevention or treatment of ischemic colitis

DNA Adhesion barrier containing natural-polymer with biocompatibility and DNA fragment mixture and process for producing the same