Showing papers by "Krystyna Klimaszewska published in 2002"
Journal Article•
TL;DR: A general description of the conifer SE process is provided, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated.
Abstract: The discovery of conifer somatic embryogenesis (SE) and the subsequent development of SE protocols for a range of genera and species have opened new research opportunities to forest biotechnologists and a means towards mass clonal propagation for the forest industry. This paper provides a general description of the conifer SE process, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated. Additionally, future research needs, including approaches for developing markers to optimize SE process and the production of high quality embryos, are discussed. Additional key words: cryopreservation, initiation, maturation, Pinaceae, somatic seedlings Addresses: K. Klimaszewska1, Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., P.O. Box 3800, Sainte-Foy, Quebec, Canada G1V 4C7. D. R. Cyr, Orycle Consulting, 801-288 E 8th Ave., Vancouver, British Columbia, Canada V5T 4S8. 1 Corresponding author: e-mail: kklimaszewska@cfl.forestry.ca, tel. (418) 648-4638, fax (418) 648-5849
44 citations
Journal Article•
TL;DR: This paper will provide a review of current efforts in conifer SE including cryopreservation, commercialization and deployment strategies, and transgenics and a discussion of challenges and issues directed at genetic fidelity, intellectual property and future needs.
Abstract: Somatic embryogenesis (SE) of conifers for clonal propagation has emerged from its earliest beginnings in 1980 to become an integral component of tree improvement strategies. With its capacity for long-term germplasm preservation and scale-up technologies, it is seen to be the preferred avenue to accelerate selection and operational deployment of value-added genotypes. At present, programs for numerous species from the Pinaceae family are underway worldwide, with activities ranging from selection trials to pilot production for plantation forestry. This paper will provide a review of current efforts in conifer SE including cryopreservation, commercialization and deployment strategies, and transgenics. A discussion of challenges and issues is directed at genetic fidelity, intellectual property and future needs. Additional key words: clonal propagation, germplasm preservation, Pinaceae, plantation forestry, somatic
36 citations