A method for fabricating a solid phase-digital PCR (Polymerase Chain Reaction) chip, comprising the following steps of: step 1, taking a substrate, removing organic and inorganic impurities on the surface of the substrate and drying the substrate in vacuum; step 2, growing a sacrificial layer on the front surface of the substrate; step 3, transferring a mask pattern to the sacrificial layer through a photoetching process and an etching process; step 4, with the sacrificial layer as the mask, forming a micropore array on the substrate through the etching process; step 5, taking a cover plate, removing organic and inorganic impurities on the surface of the cover plate and drying the cover plate in vacuum; step 6, forming a PDMS (Polydimethylsiloxane) membrane on the front surface of the cover plate through spin-coating and heating the PDMS membrane so that the PDMS membrane is cured; step 7, performing surface treatment on the PDMS membrane and bonding a DNA probe with the surface of the PDMS membrane; and step 8, bonding the substrate with the cover plate to form an independent reaction cell in each micropore in the micropore array on the substrate, thereby forming the solid phase-digital PCR chip. The method provided by the invention is simple to fabricate, easy to operate and low in cost.

Solid phase-digital PCR (Polymerase Chain Reaction) chip and method for fabricating solid phase-digital PCR chip

A micro-pillar array assembly including an inlet for receiving a biological fluid including components of the biological fluid, an outlet, and a cavity formed between the inlet and the outlet, the cavity further comprising a plurality of micro-pillar arrays positioned to remove cellular components of the biological fluid to produce a resulting fluid. Another assembly, a method and a system are also disclosed.

A micro-pillar array method and apparatus

A nucleic acid extraction device, which can realize automation, ultra-miniaturization and super-high speed in the nucleic acid extraction reaction, has no limitation to the type of biological specimens that can be used, such as sputum, blood, cells, urine, saliva, tissues, etc., minimize the used amount of the sample solution, and also maintain and/or improve the nucleic acid extraction efficiency with reliability.

Ultra-high-speed nucleic acid extracting apparatus and nucleic acid extracting method using same

A flowcell for a sequencing instrument. The flowcell includes a fluid inlet, a fluid outlet, a flow channel formed between an at least partially transparent cover and a base and fluidly connecting the fluid inlet to the fluid outlet, and a capture substrate provided in the flow channel. The capture substrate includes microretainers configured to each receive a single microspot having a microspot diameter, and microretainer is separated from adjacent microretainers by an interstitial gap distance that is equal to or greater than the microspot diameter. A particle separator may be fluidly connected to the flowcell. The particle separator may include a microfluidic channel having an array of micropillars to transfer a plurality of the microspots to a loading buffer that may be delivered to the flowcell.

Flowcells with microretainers and particle separators for discrete seeding microspots

The present invention relates to an optical sensor module for use in a PCR module which is coupled detachably to a reader system, and includes a reaction container and an optical sensor module for detecting fluorescence generated from the reaction container to amplify dielectric materials of a sample disposed in the reaction container according to the control of the reader system. The optical sensor module comprises: a base substrate; a temperature control section which is formed integrally with the base substrate and includes a plurality of temperature controlling lines containing a conductive material and elongated to generate heat according to the flow of electric current, and a resistance sensor connected to the ends of the temperature controlling lines to output the resistance among the temperature controlling lines as digital signals; and an optical sensor array which is disposed in the base substrate and has a plurality of optical sensors exposed between the adjacent temperature controlling lines and aligned in the shape of a matrix.

Photosensor module and method of manufacturing the same

PURPOSE: A micro-biochip for PCR and a method for isolating DNA using the same are provided to reduce time, cost, and the amount of sample CONSTITUTION: A micro-biochip(100) for PCR comprises a reaction tank through which sample cells are injected and a heater for controlling temperature The reaction tank has: a PCMS(Polydimethylsiloxane) chip(130) having a channel(132) with a microfilter for isolating pure DNA; a thin film glass chip(140) welded at one side of the PDMS chip The heater has glass chip(160) placed on the lower side of thin film glass chip, and micro-heater and temperature sensor(180) formed by deposition of chrome and gold on one side of the glass chip A method for isolating DNA using the micro-biochip comprises: a step of injecting microbeads to the channel; a step of filtering the microbeads on a micropillar of the channel; a step of injecting sample cells to the channel; a step of heating the sample cells for cell wall degradation; and a passing DNA through micropillar and microbeads

Micro bio chip for polymerase chain reaction and dna extracting method using the same

PURPOSE: An integrated micro biochip is provided to perform sample pre-treatment and PCR on one chip and reduce labor, cost, time and sample amount. CONSTITUTION: An integrated micro biochip(100) comprises: a sample pre-treatment reaction bath(120) for removing residues from injected sample cells; a PCR container(140) which is formed at the exit of the sample pre-treatment reaction bath and is able to perform PCR; a check valve(160) which is formed between the reaction bath and PCR container and prevents reverse flow of DNA or PCR mixture; a heating unit(180) which regulates temperature of the reaction bath and PCR container; and thin glass chip(102).

Integrated micro bio chip

PURPOSE: A microbiochip for pretreatment of sample and a method for extracting DNA using the same are provided to reduce the amount of sample, time, cost, and labor. CONSTITUTION: A micro biochip(100) for pretreatment of a sample comprises: a heating unit which regulates temperature for efficient experiment; a PDMS(polydimethylsiloxane) chip(130) having a channel(132) with a micro-filter for extracting pure DNA; and thin film glass chip(140). The heating unit comprises a glass chip(160) placed at the lower part of the thin film glass chip, micro heater, and temperature sensor(180). A method for extracting DNA using the micro biochip comprise: a step of injecting microbead to the channel in reaction bath; a step of filtering microbead in a micropillar; a step of injecting sample cells to the channel; a step of heating the sample cells with the microheater to break cell wall; and a step of extracting DNA.

Micro bio chip for sample pre-treatment and dna purification method using the same

PURPOSE: A separable thermopneumatic micropump chip with a membrane flap, and a manufacturing method thereof are provided to reduce measurement errors by preventing a sample from flowing backward. CONSTITUTION: A separable thermopneumatic micropump chip comprises a micro heater chip(10), a micro reaction chip(20), and a housing(30). The micro heater chip has a heater and a sensor formed on a substrate. The micro reaction chip is connected to a flow passage, and has an inlet, an outlet, and a pump chamber. The micro heater chip and the micro reaction chip are separated from each other. The micro reaction chip has a membrane flap which opens and closes the flow passage. The micro heater chip and the micro reaction chip are installed in the housing.

Lee Dong Ho

Papers

Micro bio chip for polymerase chain reaction and dna extracting method using the same

Integrated micro bio chip

Micro bio chip for sample pre-treatment and dna purification method using the same

Seperable thermppneumatic micropump chip employing membrane flap and fabrication method thereof