The invention discloses a preparation method of high-strength methacrylation chitosan hydrogel. The method comprises the steps that chitosan is dissolved in a solvent, methacrylic anhydride is added to obtain a methacrylation chitosan solution, dialysis is conducted on the methacrylation chitosan solution by transferring the methacrylation chitosan solution into a dialysis bag, freezing drying andcold storage are conducted, the methacrylation chitosan solution is dissolved in water, methacrylation F127 is added, a photo initiator is added to mixed liquid, uniform stirring is conducted, the hydrogel is prepared through UV photo crosslinking of 5-10 W, and the high-strength methacrylation chitosan hydrogel is obtained. The method has the advantages that the high-elasticity hydrogel with thecompression strength of 2-4 MPa can be obtained through the photo crosslinking of water soluble chitosan and F127 derivatives, and the stability of a hydrogel structure is improved.

Preparation method of high-strength methacrylation chitosan hydrogel

The invention discloses high-toughness self-repairing physical hydrogel based on dual ion coordination and a preparation method thereof. The physical hydrogel has multi-crosslinking sites. The preparation method of the high-toughness self-repairing physical hydrogel comprises the following steps: dissolving N-carboxyethyl chitosan (CEC), acrylic acid (AA), an FeCl3 water solution and a CaCl2 watersolution into de-ionized water according to a certain ratio and carrying out ultrasonic treatment to obtain a uniform and transparent solution; then carrying out vacuum deoxygenation on the solution;adding an ammonium persulfate (APS) water solution and a catalyst N,N,N',N'-tetramethyldiethylamine (TEMED) in sequence under the protection of nitrogen gas; reacting for at least 4h under the condition of 30 to 50 DEG C, so as to prepare the physical hydrogel. The physical hydrogel provided by the invention has excellent mechanical properties and self-repairing properties; metal ions are introduced so that the gel has certain ionic conductivity; furthermore, an application range of the chitosan based self-repairing hydrogel is expanded; the high-toughness self-repairing physical hydrogel ishopefully applied to fields including wearable flexible electronic devices, soft-bodied robots, biomedicines, aerospace and the like.

High-toughness self-repairing physical hydrogel based on dual ion coordination and preparation method thereof

Provided are a hydrogel anti-adhesive agent and a production method thereof. The anti-adhesive agent of the present invention includes: 0.1-10 wt% of a tyramine-modified carboxymethyl cellulose derivative; 0.5-10 wt% of pullulan; and 0.1-2 wt% of horseradish peroxidase. According to the present invention, drawbacks of an existing anti-adhesive gel has been improved, which includes adhesiveness to tissues and organs, tissue immunological response, easy manipulability, bioaffinity, and biodegradability, thereby enhancing user convenience. In addition, effective adhesiveness facilitates a fast wound healing, and the omission of anti-adhesive film removal after surgery brings a user more easier manipulability as well.

Hydrogel anti-adhesion adjuvant and manufacturing method of the same

The invention discloses a preparation method of hyaluronic acid-based double-crosslinked hydrogel, which comprises the following steps: respectively modifying hyaluronic acid to obtain furan- and dopamine-modified hyaluronic acid and furan- and phenylboronic acid-modified hyaluronic acid, blending the two modified hyaluronic acids with maleimide-terminated four-arm polyethylene glycol to generatedouble crosslinking, wherein the vicinal diol structures of phenylboronic acid and dopamine can spontaneously form phenylboronic acid ester under the condition that the pH value is 7.4 and the hydrogel has injectability and adhesion, producing single cross-linked hydrogel, and making the furan group and maleimide undergo a Diels-Alder reaction in subsequent time to form secondary cross-linking, sothat the mechanical property of the hydrogel is enhanced and the double cross-linked hydrogel is obtained. By adopting a double-crosslinking mode of click chemistry and phenylboronic acid ester, theobtained product has good mechanical properties, injectability, hyaluronidase degradation resistance and excellent biocompatibility, and the two crosslinking modes are mild and rapid and can encapsulate cells for injection.

Preparation method of hyaluronic acid-based bi-crosslinked hydrogel

The invention provides an electrically-conductive injectable hydrogel and a preparation method thereof. The preparation method comprises: oxidizing the hydroxyl group on sodium alginate (ALG) into analdehyde group to obtain partially-oxidized sodium alginate (ADA), grafting an aniline tetramer (TA) onto the partially-oxidized sodium alginate by carrying out a reaction on the aldehyde group and the amino group on the aniline tetramer to obtain an aldehyde group-containing electrically-conductive macromolecular cross-linking agent partially-oxidized sodium alginate-aniline tetramer (ADA-TA), and blending the partially-oxidized sodium alginate-aniline tetramer (ADA-TA) and gelatin to obtain the electrically-conductive injectable hydrogel. According to the present invention, the preparation method is simple, the reaction condition is mild, the biocompatibility is good, and the electric conductivity of the gel can be adjusted by controlling the grafting ratio of the aniline tetramer.

Electrically conductive injectable hydrogel and preparation method thereof

Injectable double network hydrogels and biomedical use thereof

The present invention relates to a production method of hydrogel which comprises the following steps producing an enzyme-immobilized supporter; contacting a polymer with a phenol or aniline functional group on a side chain and the enzyme-immobilized supporter under the presence of hydrogen peroxide for crosslinking; and obtaining the hydrogel by separating the enzyme-immobilized supporter. According to the present invention, the polymer with the phenol or aniline functional group on the side chain is crosslinked by the enzyme immobilized on the supporter to form the hydrogel, thereby obtaining the hydrogel without enzymes. Therefore, in vivo stability including an immune reaction by enzymes can be overcome, and the biomedical application range of the hydrogel can be expanded. [Reference numerals] (AA) Oxidizing enzyme-immobilized supporter; (BB,GG) In situ formation hydrogel; (CC) Injection needle; (DD) Enzyme-immobilized supporter; (EE) Polymer solution sterilizing filter; (FF) Polymer solution(in H_2O_2)

Preparation method of in situ forming hydrogel using enzyme-immobilized supports and biomedical use thereof

The present invention relates to a cell culturing substrate having a biopolymer pattern formed thereon, a method for producing the same, and a method for producing and delivering a micro-sized cell sheet through the same The cell culturing substrate according to the present invention can freely adjust the shape of a cell sheet by local-specifically inducing the adsorption of cells according to a polycatechol-based polymer pattern formed on temperature-sensitive hydrogel which reversibly showing expansion and shrinkage according to a change in a temperature Thus, the cell culturing substrate can easily imitate the structure and the function of complicated human body organs, and can maintain the shape and the function of a cell sheet The cell sheet is separated from the hydrogel in a short time, and is simultaneously delivered to a target Accordingly, the present invention can be applied to a medical cell delivery treatment system and a cell-based regenerative medicine artificial tissue transplanting materials

Substrate with biopolymer pattern for culturing micro-sized cell sheet and method for delivering the same

The present invention relates to pharmaceutical compositions for liver protection comprising keratin, PEGylated keratin or PEGylated keratin nanoparticles and pharmaceutical compositions for prevention or treatment of hepatotoxic diseases. The keratin, PEGylated keratin or PEGylated keratin nanoparticles have low toxicity or no toxicity, significantly elevate the expression of UGT1A1 and related receptors, and have hepatocyte protective action and hepatotoxicity inhibiting action, thereby being usefully used as a composition for liver protection or a composition for prevention or treatment of hepatotoxic diseases.

Complex nanoparticle comprising PEGylated keratin with relieving side effects of drugs method for preparing the same and transporter carrying and releasing drugs using the complex nanoparticle

The present invention relates to a multilayer cardiac stem cell sheet and a method for manufacturing same and aims to provide a method for manufacturing a multilayer cell sheet by embedding cardiac stem cells into a hydrogel using a biodegradable natural polymer hydrogel as a three-dimensional support, and subjecting same to three-dimensional monoculture. The multilayer cell sheet, according to the present invention, does not require special tools to manufacture, is easy to handle due to good physical properties, increases cell viability after a transplant due to ample accumulation of various growth and protection factors and extracellular matrix between cells, and transportation of nutrition is made easy due to thinness owing to shrinkage caused by the cells. As a result, the multilayer cardiac stem cell sheet is expected to be useful as a myocardial regeneration agent.

Lee Yun Ki

Papers

Injectable double network hydrogels and biomedical use thereof

Preparation method of in situ forming hydrogel using enzyme-immobilized supports and biomedical use thereof

Substrate with biopolymer pattern for culturing micro-sized cell sheet and method for delivering the same

Complex nanoparticle comprising PEGylated keratin with relieving side effects of drugs method for preparing the same and transporter carrying and releasing drugs using the complex nanoparticle

Multilayer cardiac stem cell sheet and method for manufacturing same