The invention discloses a method for preparing obeticholic acid from a new derivative of 3alpha-hydroxy-7-oxo-5beta-cholanic acid. The synthesis method comprises the following steps: firstly, carrying out hydroxyl and carboxyl protection on the 3alpha-hydroxy-7-oxo-5beta-cholanic acid to prepare the corresponding new derivative; secondly, obtaining the obeticholic acid respectively according to two synthesis routes. According to the method disclosed by the invention, a safer protecting group reagent is utilized, and the problem that ultraviolet absorption of an intermediate is not strong is solved; the intermediate is easier to purify, the yield is improved, and the cost is reduced, so that the method is more suitable for industrialized amplification, and has remarkable creativity and actual application value.

Method for preparing obeticholic acid from new derivative of 3alpha-hydroxy-7-oxo-5beta-cholanic acid

The invention provides an application of 1,8-naphthyridinyl fluorescence molecular probes in the study of entertainment abuse neuromechanism of ketamine and the diagnosis of the addiction level. The study of the in-vivo drug addiction mechanism through using a fluorescence imaging technology is not reported home and abroad, the study of the neuromechanism does not appear in the existing reports ofthe entertainment abuse of the ketamine, and the neuromechanism deduction of entertainment abuse living bodies of the ketamine, treatment target searching and the visual study of the diagnosis of theaddiction level are realized in the invention.

Application of 1,8-naphthyridinyl fluorescence molecular probes in study of entertainment abuse neuromechanism of ketamine and diagnosis of addiction level

The present invention relates to a colorimetric fluorescent probe for ultrasensitive analysis of mercury based on resorufin Specifically, the probe is a class of resorufin-like compounds and can be used as a mercury ion colorimetric fluorescent probe for detection of mercury ions The probe can achieve at least one of the following technical effects: high-selective identification of the mercury ions, ultrasensitive analysis of the mercury ions, stable property, long-term preservation and use and relatively strong anti-interference ability

Colorimetric fluorescent probe for ultrasensitive analysis of mercury based on resorufin

The invention relates to a rapid high-selectivity ultra-sensitive carbon oxide ratio fluorescent probe. Specifically, the probe is made of allyl ether compounds and can be used as the carbon oxide ratio fluorescent probe for detecting carbon oxide. The probe has at least one technical effect of highly selectively recognizing carbon oxide, rapidly responding to carbon oxide, highly sensitively analyzing carbon oxide, analyzing the ratio of carbon oxide, being stable in property and capable of being stored and used for a long time, and being high in anti-interference capacity.

Rapid high-selectivity ultra-sensitive carbon oxide ratio fluorescent probe

The invention belongs to the field of specific molecular recognition materials and discloses a cyanine fluorescent compound as well as a preparation method and application thereof. The structural general formula of the cyanine fluorescent compound is shown as a formula (I). The fluorescent compound disclosed by the invention adopts a classic cyanine dye structure, an electroneutral electron-drawing group is introduced into the R-group position, a conjugated structure of push-pull electron interaction is formed between the electron-donating group and the electron-drawing group in the structure,and the conjugated system for transition is increased by carbon-carbon double bonds, so that fluorescence produced by compound molecules moves to a near infrared region. Meanwhile, due to the introduced electroneutral group, the problem that most of the charged cyanine compounds cannot pass through the blood brain barrier is solved. The compound molecule has affinity to Abeta plaques, can be successfully applied to near infrared fluorescence imaging of the Abeta plaques, and has the advantages of being safe, non-radioactive, low in cost, low in background fluorescence interference, high in penetrating power in biological tissues and the like. The structural formula is as shown in the specification.

Cyanine fluorescent compound as well as preparation method and application thereof

The invention belongs to the technical field of detection materials and discloses a CO probe and a preparation method and application thereof. The CO probe has a structural formula shown as the formula (I). According to the preparation method, chloro-paranitrobenzo furazan is added into an n-propylamine organic solution, then anhydrous potassium carbonate is added, the mixture is stirred at room temperature for a reaction, and the reaction product is subjected to separation and purification to obtain 4-propylamino-7-nitro-2,1,3-benzooxadiazole; 4-propylamino-7-nitro-2,1,3-benzo oxadiazole is dissolved in an anhydrous organic solvent, diisopropylethylamine is added under protection of an inert atmosphere, the mixture is stirred and mixed to be uniform, then allyl chloroformate is added dropwise for a reaction at room temperature, and the reaction product is subjected to separation and purification to obtain the CO probe. Through the preparation method, the process is simple, and operation is easy; the obtained CO probe responds to both ultraviolet and fluorescent light and can be applied to the field of CO detection. (Please see the description for the formula (I).).

CO probe and preparation method and application thereof

The invention belongs to the technical field of heavy metal detection and discloses an organic small-molecule probe for detecting zero-valent palladium and a preparation method thereof. The chemical structure of the organic small-molecule probe is shown in the formula (I). The preparation method includes the steps that resorufin sodium salt, an organic solvent and triethylamine are stirred and mixed to be uniform, then allyl chlorocarbonate is added dropwise, extraction is conducted after a room temperature reaction, an organic phase is concentrated, and then the organic small-molecule probe for detecting zero-valent palladium can be obtained. The organic small-molecule probe is synthesized only through one step, the aftertreatment process is simple, operation is easy, and products are easy to obtain. Ultraviolet and fluorescence dual-response detection of heavy metal palladium in a pure water phase system can be achieved, the advantages of being rapid in response and high in specificity are achieved, and wide application prospects are achieved in the fields of chemical engineering, environments, biomedicine and the like. The formula (I) is shown in the description.

Organic small-molecule probe used for detecting zero-valent palladium and preparation method thereof

The invention belongs to the technical field of transition metal detection and discloses a fluorescence probe for detecting three-valent iron ions and a preparation method of the fluorescence probe. The structural formula of the fluorescence probe is represented by a formula (I) (shown in the description). The preparation method comprises the following steps: reacting by virtue of rhodamine B andhydrazine hydrate in an ethanol solution, so as to obtain an intermediate 1; reacting by virtue of the intermediate 1 with chloroacetyl chloride and triethylamine in dichloromethane under an ice bathcondition, so as to obtain an intermediate 2; and adding potassium iodide, 2-methoxy-benzylamine or 2-thienylmethylamine and potassium carbonate into acetonitrile for reaction, so as to obtain a fluorescence probe for detecting Fe . The preparation method only comprises the three steps, the after-treatment process is simple, the operation is simple, and the product is easily prepared; and the prepared product can be applied to ultraviolet and fluorescent double response reaction detection of Fe and can be used for detecting Fe through naked eyes; and the preparation method has the advantages of high response speed and sensitivity and has wide application prospects in the fields of chemical engineering, environment, biological medicines and the like.

Fluorescence probe for detecting three-valent iron ions and preparation method of fluorescence probe

The invention belongs to the technical field of heavy metal detection and discloses a near-infrared fluorescent probe for detecting zero-valence palladium and a preparation method of the near-infrared fluorescent probe. The chemical structural formula of the near-infrared fluorescent probe is shown in formula (I). The preparation method of the near-infrared fluorescent probe comprises steps as follows: methylene blue, water, an organic solvent, sodium bicarbonate and sodium hydrosulfite are uniformly mixed, react at 40-60 DEG C for 2-4 h and are cooled to the room temperature, liquid separation is performed, and an organic solvent layer is reserved; potassium carbonate is added under the water bath condition, allyl chloroformate is dropwise added, extraction is performed after the reaction at the room temperature, an organic phase is concentrated, and a product is obtained through column chromatography purification. The synthesis method is simple and easy, and the obtained product can realize ultraviolet and near-infrared fluorescent dual-responsive detection of heavy metal palladium in a pure aqueous phase system, has the advantages of response rapidness and high specificity and has broad application prospect in chemical, environment, biomedicine and other fields.

Near-infrared fluorescent probe for detecting zero-valence palladium and preparation method of near-infrared fluorescent probe

The invention belongs to the technical field of pharmaceutical synthesis, and discloses a preparation method for 3[alpha],7[alpha]-dihydroxyl-6[alpha]-ethyl cholanic acid. The method comprises the steps: with 3[alpha]-hydroxyl-7-carbonyl cholanic acid as a raw material, carrying out methyl esterification, and under alkaline and low temperature conditions and with estersil as a catalyst, carrying out a reaction with halogenated silane to obtain 3[alpha],7-di(trimethyl siloxy)-6-en-methyl cholanate; then under conditions of low temperature and boron trifluoride acetonitrile, carrying out a reaction with Mukaiyama aldol, to obtain 3[alpha]-hydroxyl-6-ethidene-7-carbonyl methyl cholanate; and then with palladium carbon as a catalyst, carrying out catalytic hydrogenation and deprotection reaction in an alkaline alcohol/aqueous solution, acidifying to obtain 3[alpha]-hydroxyl-6[alpha]-ethyl-7-carbonyl cholanic acid; and finally reducing with sodium borohydride to obtain the target product. The method has the advantages of simple synthesis process, mild conditions and high yield, and is suitable for industrialization.

Li Jing

Papers

CO probe and preparation method and application thereof

Organic small-molecule probe used for detecting zero-valent palladium and preparation method thereof

Fluorescence probe for detecting three-valent iron ions and preparation method of fluorescence probe

Near-infrared fluorescent probe for detecting zero-valence palladium and preparation method of near-infrared fluorescent probe

Preparation method for 3[alpha],7[alpha]-dihydroxyl-6[alpha]-ethyl cholanic acid