The invention belongs to the field of microorganisms and in particular relates to a brilliant green lactic acid medium The medium comprises the following components: EC broth, 20g/L of bile salt, 0012g/L of bright green, 30g/L of manganese sulfate and 150g/L of agar, wherein the pH value of the medium is 71-73; the EC broth per liter contains 200g of tryptone, 100g of lactose, 20g of potassium dihydrogen phosphate, 50g of sodium chloride, 15g of bile salt No3, 40g of dipotassium hydrogen phosphate and the balance of distilled water The brilliant green lactic acid medium has the beneficial effects that the medium is simple in components, convenient in preparation operation and low in cost and is beneficial to popularization and application; the bacteria grow rapidly and the mutation rate is low; the medium is convenient to observe and analyze

Brilliant green lactic acid medium and application thereof

The invention belongs to the field of microbes and relates to a brilliant green lactic acid medium. The brilliant green lactic acid medium comprises EC broth, 2.0g/L of cow bile salt, 0.012g/L of brilliant green, 3.0g/L of manganese sulfate and 15.0g/L of agar. The brilliant green lactic acid medium has a pH value of 7.1-7.3. Each liter of the EC broth comprises 20.0g of tryptone, 10.0g of lactose, 2.0g of monopotassium phosphate, 5.0g of common salt, 1.5g of bile salt NO.3, 4.0g of dipotassium hydrogen phosphate and the balance distilled water. The brilliant green lactic acid medium has a simple formula, can be prepared conveniently, has a low cost, is conducive to popularization application, promotes bacterium fast growth, reduces a variation rate and is convenient for observation and analysis.

Liu Hanbin

Papers

Brilliant green lactic acid medium and use thereof