Objective: This study aimed to separate and pure chitinase producing actinomycetes with stronger activity from lawn soils of Beijing. Method: Morphology and culture characteristics were observed. physiological and biochemical characteristics were detected. 16SrDNA sequence was analyzed. Result: The highest chitinase activity strain was Bn035, whose relative diameter of transparent circle K2 was 3.42 at which the chitinase activity of fermentation broth reached a maximum value of 1.23 U/mL in the 6th day. Crude enzyme solution and bacteria cake of strain Bn035 generated stronger bacterial resistance to all root rot fungi which indicated that the strain had a strong broad-spectrum resistance to root rot fungi. Conclusion: The strain was identified as Streptomyces scabrisporus. It will be a potential biological control function.

https://file.scirp.org/pdf/JBM_2015031814523993.pdf

Screening and Identification of Chitinolytic Actinomycetes and Study on the Inhibitory Activity against Turfgrass Root Rot Disease Fungi

The effect of bacteriostatic such as penicillin,norfloxacin,nalidixic acid,potassium dichromate and tatin were studied individually.The orthogonal design was used in this experiment and the software STATISTICA6.0 was employed to analyse the result.The result showed that the potassium dichromate was the best bacteriostatic among those bacteriostatices.The best bacteriostatics assembly was potassium dichromate 50 mg·L-1,nalidixic acid 10 mg·L-1,norfloxacin 10 mg·L-1.More over,nalidixic acid was the most important among the three bacteriostatices.

LU Kai-feng

Papers

Effect of Bacteriostatic on the Isolation of Actinomycetes