Pathophysiology of Campylobacter enteritis

https://www.researchgate.net/profile/Dirk_Hofreuter/publication/250923959_Molecular_methods_to_investigate_adhesion_transmigration_invasion_and_intracellular_survival_of_the_foodborne_pathogen_Campylobacter_jejuni/links/00b4952298aac37c83000000.pdf

Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni

Plasmid pUA466, a 45-kilobase transmissible tetracycline resistance plasmid from Campylobacter jejuni was mapped with AvaI, AvaII, BclI, HincII, PstI, XhoI, and XbaI. The resistance determinant was cloned and expressed in Escherichia coli and was homologous with a class M determinant from Streptococcus spp.

Plasmid-mediated tetracycline resistance in Campylobacter jejuni: expression in Escherichia coli and identification of homology with streptococcal class M determinant.

The ability of Campylobacter coli and Campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. MIC data for C. coli indicated that some strains were more susceptible to the antibiotics than were the C. jejuni strains tested. A reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. Only one of nine of the antibiotic combinations studied did not markedly inhibit most of the C. coli strains tested. Although one C. coli strain was not inhibited by any of the antibiotic combinations, the other six strains were inhibited for up to 7 log cycles. The addition of blood or growth supplements reduced but did not eliminate the inhibitory effect. The inhibition of laboratory strains of C. coli on media developed for the isolation of Campylobacter spp. indicates that the incidence of C. coli may be underestimated.

Inhibition of Campylobacter coli and Campylobacter jejuni by antibiotics used in selective growth media.

Campylobacter jejuni is a fastidious organism, growing in microaerophilic conditions with a temperature range between 37 degrees and 42 degrees C. Multiple types of media can be used to cultivate it; however, Mueller Hinton broth and agar support the best C. jejuni growth. Optimum atmosphere for C. jejuni is 85% N2, 10% CO2, and 5% O2.

/pdf/growth-and-laboratory-maintenance-of-campylobacter-jejuni-1tj9hrxr6o.pdf

Growth and Laboratory Maintenance of Campylobacter jejuni

Four strains of Campylobacter jejuni and four strains of Campylobacter coli were used to compare the quantitative growth of Campylobacter cells on blood agar base no. 2 (Oxoid), brucella agar (BBL Microbiology Systems and Difco Laboratories), campylobacter agar base (Difco), Columbia blood agar base (Difco and Oxoid), and Mueller-Hinton agar (Difco and Oxoid). Columbia blood agar base and blood agar base no. 2 were inhibitory to most of the strains tested, as evidenced by reduced (10- to 1,000-fold) colony counts compared with other basal media. One of the brucella agars was inhibitory to two of the C. coli strains. The inhibitory effect of these media could be eliminated by addition of FBP (0.05% each ferrous sulfate hydrate, sodium metabisulfite, and sodium pyruvate) or 7% defibrinated sheep blood. However, addition of FBP or blood to brucella agar, campylobacter agar base, or Mueller-Hinton agar did not significantly affect the count, indicating that supplements are not required in these media for growth of Campylobacter in pure culture.

https://jcm.asm.org/content/jcm/21/2/226.full.pdf

M E Stiles

Papers

Comparison of basal media for culturing Campylobacter jejuni and Campylobacter coli.