M. Van Lookeren Campagne

TL;DR: This study shows that dehydration by freeze-substitution, combined with Lowicryl HM20 embedding at sub-zero temperature, provides a successful method of preparation of fixed brain tissue for ultrastructural studies, allowing immunogold localization of several neural antigens by double labeling in the same section and in serial sections.

TL;DR: The similar B-50 localization in neuronal growth cones and synaptosomes suggests that, both in extending axons and mature synaptic terminals, B- 50 may exert identical functions as a protein kinase C substrate at the plasma membrane.

TL;DR: In situ end-labeling (ISEL) identifies DNA fragmentation in apoptotic or necrotic nuclei in tissue sections as discussed by the authors, but it requires conservation of DNA integrity during the postmortem delay (PMD) and good accessibility of fragmented DNA after (prolonged) tissue fixation.

TL;DR: During development of the stratum radiatum in the hippocampal field CA1, the localization of B-50 persists at the plasma membrane of axons and axon terminals in P1 and P90 rats, consistent with the suggestion that B- 50 acts as a regulator of neurotransmitter release and intracellular messengers.

TL;DR: It is suggested that at this stage of development B-50 does not participate directly in the processes leading to morphological polarization, suggesting a role of these vesicles in axonal transport, membrane insertion and (or) recycling.