The invention discloses an inducing method for murraya paniculata callus. The inducing method comprises the following steps: S1) pre-treating materials: using 75% ethanol solution for disinfecting mature murraya paniculata seeds for 1-2min, and then using mercury bichloride containing Tween 20 for disinfecting seeds for 6-12min, removing seed coats from the disinfected seeds, and inoculating intoa MS minimal medium for culturing aseptic seedlings; S2) inducing callus: taking blades or root segments of aseptic seedlings as explants, and inoculating into an induction medium for culturing till growing callus. According to the invention, the blades or root segments of aseptic seedlings are taken as explants; the induction medium suitable for callus of blades or root segments is respectively researched; the inductivity of murraya paniculata callus can be increased while the browning rate is reduced; the subsequent proliferation subculture of callus is not influenced; the inducing method can be applied to murraya paniculata seedling propagation, polyploid induction, mutation induction and transgenosis germplasm innovation.

Inducing method for murraya paniculata callus

The invention relates to a cultivation method for vegetable-type telosma cordata. The cultivation method comprises the following steps of A, seedling growing, wherein a cuttage seedling growing mode is adopted, and half-lignification stem vines which grow robustly are selected, cut into cuttings and aslant inserted in a nutrient bag so that the stem vines sprout and grow into seedlings; B, field planting, wherein a planting garden is selected, ditching is conducted to form high ridges, the seedlings obtained in step A are planted in each piece of ridge block, and the seedlings are covered withgrass and sprayed with water after field planting; C, fertilization management, wherein an aqueous compound fertilizer solution is sprayed after new buds are grown out of field-planted plants; D, erection of a trellis and guiding of vines, wherein when the stem vines of the seedlings which are subjected to field planting grow to 80-100 cm, erection of the trellis and guiding of the vines are conducted; E, prevention and control of diseases and insect pests, wherein mites and scale insects are prevented and controlled, and the wilt is prevented and treated; F, harvesting, wherein when 40-50% of florets on inflorescences are about to blossom, the whole inflorescences can be picked. The cultivation method for planting telosma cordata is easy in management, low in cost, and high in yield; theeconomic benefit is greatly increased, and the method is suitable for being widely popularized for planting.

Cultivation method for vegetable-type telosma cordata

The invention discloses a cutting seedling method of murraya paniculata. The cutting seedling method includes the steps of 1), clipping stems and branches of the murraya paniculate to obtain cutting slips 8-15cm in length and retaining at least two sections; 2), soaking lower cuts in water and a plant growth regulator sequentially; 3), plugging the cutting slips treated in the step 2) in soil and keeping air humidity of the grow environment to be 75wt% to 85wt%; 4), enabling the cutting slips in the step 3) to take root under light shading percentage of 70-80% at the temperature ranging 15DEG C-30DEG C, transplanting seedlings taken root to seed beds for cultivation, and after the seedlings grow up to 35cm, taking the seedlings out of the seed bed and performing field planting. The cutting seedling method has the advantages of short rooting cycle, high adaption, simple cultivation process and high breeding coefficient, purposes of fast growth, stable yield and high yield can be achieved, harvesting years are reduced, and sustainable and healthy development of wild murraya paniculata resources is sustained.

Cutting seedling method of murraya paniculata

Belonging to the technical field of plant propagation, the invention in particular relates to a rapid propagation method for murraya paniculata. The method includes the steps of: (1) taking murraya paniculata seed, a stem segment with terminal bud or stem segment with axillary bud as the explant, conducting pretreatment and disinfection treatment to obtain a disinfected explant; (2) inoculating the disinfected explant into an initial medium for culture to obtain an explant with new shoot; (3) inoculating the explant with new shoot into a proliferation medium for culture to obtain rootless seedlings; and (4) inoculating the rootless seedlings into a rooting medium for culture so as to obtain seedlings, wherein the rooting medium is obtained by adding 8g/L agar powder, 30g/L sucrose, 0-1.5mg/L IBA and 0-1.5mg/L NAA into a 1/2MS medium, and has a pH of 5.8-6.0. The method can realize the rapid proliferation of murraya paniculata, and the obtained seedlings have good quality.

Rapid propagation method for murraya paniculata

The invention relates to the field of natural pharmaceutical chemistry, and particularly provides a coumarin dimer compound, a pharmaceutical composition and a preparation method and application of the coumarin dimer compound. The coumarin dimer compound has a structure shown as a formula I shown in the specification. The coumarin dimer compound can remarkably improve nerve cell damage of an A[beta]1-42 induced senile dementia model; moreover, can improve neurotrophic factor expression, has anti-inflammatory and antioxidant effects, and can be used for preventing and treating nervous system degenerative diseases such as vascular dementia, vascular cognitive impairment, senile dementia (also called Alzheimer's disease), cholinergic neurodegenerative change, learning and memory ability decline and the like.

Coumarin dimer compound, pharmaceutical composition, and preparation method and application of the coumarin dimer compound

The invention belongs to the technical field of wild chrysanthemum cultivation, in particular to a wild chrysanthemum planting method. According to the method, wild chrysanthemum flower seeds are creatively directly sowed or drill-sowed in a wetland field, the wetland can ensure that the surface soil is moist for a long time, so that the seeds are easy to absorb water, and the problem of water shortage does not occur in the seed roots after the seeds absorb water and germinate is solved, the phenomenon that the seeds are sun-dried or dry after absorbing water and swelling is avoided, and the problem that the seeds are difficult to germinate and absorb water is solved; if the seeds are sown in wetland and covered with soil, with the seed weight of chrysanthemum indicum being only 0.1g, cotyledons are difficult to excavate; and by arranging drainage ditches in the wetland, the drainage property of the wetland can be ensured, the problem of stagnant water and dead seedlings cannot occur during the growth of the wild chrysanthemum, the wild chrysanthemum can normally grow, and the survival rate of the wild chrysanthemum is ensured.

Ma Penggang

Papers

Cutting seedling method of murraya paniculata

Rapid propagation method for murraya paniculata

Coumarin dimer compound, pharmaceutical composition, and preparation method and application of the coumarin dimer compound

Wild chrysanthemum planting method