The invention relates to a method for testing content of menthol in an acetate fiber filter stick through matrix matching correction-headspace gas chromatography. The method comprises the following steps: 1) preparing an interior label liquid, a standard storage liquid and a standard working solution; 2) pretreating a sample, namely, peeling off cut tobacco and paper from a cigarette sample, putting a filter tip into a headspace bottle of 20 mL, adding the interior label liquid, sealing by using a gland and subsequently feeding the sample; 3) testing the sample by using an Agilent headspace gas chromatographic instrument; 4) determining the nature and quantifying, namely, determining the nature according to a standard sample retaining time, a single fed standard sample and a chromatogram of a blank solution, the standard solution and a practical sample solution, quantifying by using an internal label method, and drawing a standard working curve by using a peak area ratio y and a concentration ratio of the menthol to the internal label to obtain corresponding retaining time, a linear equation, detected limit and detection limit. The method adopts headspace sample feeding, the pretreatment on the sample is simple and easy to realize automation, and the method has the advantages of simplicity, rapidness, accuracy, automation, wide application range and the like.

Method for testing content of menthol in acetate fiber filter stick through matrix matching correction-headspace gas chromatography

PROBLEM TO BE SOLVED: To provide a partitioning member which can easily package commodities having different sizes at a low cost SOLUTION: In this partitioning member made of a cardboard which is fitted in an external box B, a pair of side receiving walls 1 extending along the side walls of the external box B are formed by connecting an internal receiving plate 3, a top plate 4 and an external receiving plate 5 in order Then, the internal receiving plates 3 of the side receiving walls 1 are connected with each other with a bottom superposing plate 9 Then, a partitioning wall 2 is extended from the end edge of the bottom superposing plate 9 By the side receiving walls 1, the bottom raising and the fixation of cans P and Q can be performed Therefore, other members such as a spacer are not required to be set in the external box B

Partitioning member made of cardboard

Cloth controller for sewing machine

PROBLEM TO BE SOLVED: To provide a pedal device of a keyboard musical instrument that can facilitate adjustments and maintenance and increase a degree of freedom of design. SOLUTION: The pedal device includes a chassis 3 which has a hollow pedal fitting portion 26 and is grounded to a floor F, the pedal fitting portion 26 being provided with a fulcrum part 33 at the rear part and having a window part 32 formed at its front part; a pedal 2 which has its rear part stored in the pedal fitting part 26 of the chassis 3 and rotatably supported on the fulcrum part 33 and also has its front part projected forward through the window part 32 to be stepped down; and a cover 4 which has its reverse surface formed in a hollow shape and is detachably fitted to the chassis 3 from above to cover the pedal fitting portion 26, a window part 36 which is open downward and through which the pedal 2 runs being formed on a front wall 4b. COPYRIGHT: (C)2008,JPO&INPIT

Pedal device of keyboard musical instrument

The invention provides a method for determining menthol content in cigarette filter capsules. The method comprises the following steps: preparing an internal standard solution; preparing a standard solution; preparing a sample solution; and determining the menthol content by headspace gas chromatography. The invention uses non-toxic triacetin as a matrix calibrator for menthol determination, the experimental result is accurate and reproducible, which has the advantages of simple operation, rapidity and high sensitivity.

Method for determining menthol content in cigarettes filter capsule

PURPOSE:To efficiently produce industrially useful l-menthol, by bringing a strain obtained by cultivating a specific bacterium belonging to the genus Cellulomonas or a crushed strain thereof, etc., into contact with l-menthone. CONSTITUTION:Cellulomonas turbata sp. KE31 (FERM 9059) is cultivated in a liquid culture medium containing 0.1-5% at least one of D-arabinose, L- arabinose, maltose, galactose and sodium gluconate and yeast extract or corn steep liquor at 25-36 deg.C, preferably by successively adding 0.02-0.05% l- menthone [ethanol:l-menthone = 1:1 (vol./vol.)] at the start of the cultivation and every 6-24hr thereafter for 1-4 days to afford the aimed l-menthol. Cultivated microbial cells, obtained by the above-mentioned cultivation method and having the ability to produce the l-menthol, crushed microbial cells thereof or an active fraction purified therefrom can also be brought into contact with l-menthone to provide the aimed l-menthol.

Production of l-menthol

PURPOSE:A microorganism in Cryptococcus is cultured and a novle aldose reductase is obtained from the culture mixture CONSTITUTION:Cryptococcus lactacivorus sp H131 (FERM 7840) is cultured in a medium containing appropriate amounts of yeast essence and xylose or the microorganism is cultured with the yeast essence and glucose and cultured again in a medium to which xylose and ribose are added An aldose reductase A is isolated uniformly from the culture mixture by ammonium sulfate fractionation, ion-exchange chromatography and other techniques

Production of novel aldoses reductase with microorganism

PURPOSE:To produce sorbitol in high efficiency, by contacting glucose with a product obtained by culturing a specific microbial strain capable of producing sorbitol CONSTITUTION:A microbial strain belonging to Azotobacter genus, Bejerinckia genus or Mycobacterium genus and capable of producing sorbitol is cultured in a medium containing a pentose as a main carbon source The obtained cultured product is made to contact with glucose to obtain sorbitol Any commercially available microbial strain can be used for the purpose, and the strain of Azotobacter genus is preferably Azotobacter vinelandii IAM1078 The preferable strain of Bejerinckia genus is Bejerinckia indica IAM1195, and that of Mycobacterium is Mycobacterium phlei IAM12064

Production of sorbitol by microorganism

PURPOSE:A microorganism in Cryptococcus is cultured and a novel aldose reductase which has ability to reduce ketoses and can utilize NADPH and NADH as a coenzyme CONSTITUTION:Cryptococcus lactacivorus sp H131 (FERM 7840) is cultured in a medium containing apropriate amounts of yeast essence and xylose, or cultured first with a medium containing yeast essence and glucose, then cultured again with medium containing xylose and ribose A uniform aldose reductase A is obtained by ammonium sulfate fraction, ion-exchange chromatography and other techniques The resultant aldose reductase has ability to reduce ketoses and can utilize NADH or NADPH

Novel aldose reductase

PURPOSE:To produce 3alpha-hydroxysteroid dehydrogenase having high stability, by cultivating a microorganism, belonging to the genus Cellulomonas and having the ability to produce the 3alpha-hydroxysteroid dehydrogenase having specific properties. CONSTITUTION:A microorganism, belonging to the genus Cellulomonas and having the ability to produce 3alpha-hydroxysteroid dehydrogenase having the following characteristics is cultivated. With reductive ability for steroid compounds having oxo group at the 3-position and carbonyl group-containing compounds, 98,000 molecular weight measured by a native polyacrylamide electrophoresis, 24,000 molecular weight measured by sodium dodecyl sulfate (SDS) electrophoresis and stable when allowed to stand at pH6-8 (1/15M phosphoric acid buffer solution) and 30 deg.C for 24hr and pH7 and 60 deg.C for 2hr. The aimed 3alpha- hydroxysteroid dehydrogenase is then collected from the culture thereof.

Maeda Hidekatsu

Papers

Production of l-menthol

Production of novel aldoses reductase with microorganism

Production of sorbitol by microorganism

Novel aldose reductase

Novel 3alpha-hydroxysteroid dehydrogenase and production thereof