Showing papers by "Manuela M. Pereira published in 2012"
TL;DR: It is observed that HCOs are widely distributed in the two prokaryotic domains and that the different types of enzymes are not confined to a specific taxonomic group or environmental niche.
161 citations
TL;DR: A historical perspective on the investigation of ACIII is presented, followed by an overview of the present knowledge on these enzymes, and several hypotheses for energy conservation modes are raised including linear and bifurcating electron transfer pathways.
49 citations
TL;DR: A hypothesis is put forward to explain some apparently contradictory data on the nature of the coupling ion, and the role of H+ and Na+ cycles in the overall bioenergetics of the cell is revisited.
Abstract: Summary Respiratory complex I plays a central role in energy transduction. It catalyzes the oxidation of NADH and the reduction of quinone, coupled to cation translocation across the membrane, thereby establishing an electrochemical potential. For more than half a century, data on complex I has been gathered, including recently determined crystal structures, yet complex I is the least understood complex of the respiratory chain. The mechanisms of quinone reduction, charge translocation and their coupling are still unknown. The H 1 is accepted to be the coupling ion of the system; however, Na 1 has also been suggested to perform such a role. In this article, we address the relation of those two ions with complex I and refer ion pump and Na 1 /H 1 antiporter as possible transport mechanisms of the system. We put forward a hypothesis to explain some apparently contradictory data on the nature of the coupling ion, and we revisit the role of H 1 and Na 1 cycles in the overall bioener
14 citations
TL;DR: The relation of some complexes I with Na(+) is addressed and an innovative methodology using (23)Na NMR spectroscopy is developed, which allowed the investigation of Na(%) transport taking the advantage of directly monitoring changes in Na(+) concentration.
10 citations
TL;DR: Tissue specific metabolic consequences of isolated mitochondrial complex I deficiency in mice G. Manjeri, S. Roelofs, A. Janssen, L. Blanchet, J. Driessen, R. Rodenburg, and P. Smeitink .