Showing papers by "Marc-Henri Stern published in 1994"

The MTCP-1/c6.1B gene encodes for a cytoplasmic 8 kD protein overexpressed in T cell leukemia bearing a t(X;14) translocation.

Abstract: The t(X;14)(q28;q112) translocation is associated with mature T-cell proliferations Recently this translocation has been shown to implicate the MTCP-1/c61B gene on chromosome Xq28, leading to aberrant or overexpressed MTCP-1 transcripts The potential coding role of this gene was made uncertain by the lack of a long open reading frame in its major transcripts However, a short 204 bases open reading frame is potentially coding for a 68 amino-acid protein Here, we show that this open reading frame sequence and the deduced product are well conserved in mouse A 8 kD protein (p8), which corresponds to the predicted molecular weight was revealed in transient transfectants and in cell lines by Western blotting, using a rabbit antiserum This product was absent in lymphoblastoid cell lines with deletions of the MTCP-1/c61B locus A dramatic overexpression of p8 was found in leukemic cells from a patient with a t(X;14) This small protein was localized in the cytoplasm by immunofluorescence In conclusion, MTCP-1 encodes for a cytoplasmic 8 kD product Its potential role in leukemogenesis is supported by its overexpression in leukemia with t(X;14), but its function remains unknown