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Mark Anton Jensen
Researcher at DuPont
Publications - Â 18
Citations - Â 2098
Mark Anton Jensen is an academic researcher from DuPont. The author has contributed to research in topics: Spacer DNA & Genetic marker. The author has an hindex of 10, co-authored 18 publications receiving 2073 citations.
Papers
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Journal ArticleDOI
A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides
James Merrill Prober,George L. Trainor,Rudy Johan Dam,Frank W. Hobbs,Charles William Robertson,Robert J. Zagursky,Anthony Joseph Cocuzza,Mark Anton Jensen,Kirk Baumeister +8 more
TL;DR: A DNA sequencing system based on the use of a novel set of four chain-terminating dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye distinguished by its fluorescent emission, and the sequence analysis of M13 phage DNA made with this system is described.
Journal ArticleDOI
Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms.
TL;DR: In this paper, a unified set of primers and polymerase chain reaction conditions was developed to amplify spacer regions between the 16S and 23S genes in the prokaryotic rRNA genetic loci.
Journal ArticleDOI
Effect of PCR conditions on the formation of heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions.
TL;DR: Heteroduplex and single-stranded DNA structures were generated by denaturing and reannealing spacer amplification products in the absence of polymerase activity and the mobility of heterod uplex andsingle-strander DNA structures DNA structures is extremely sensitive to electrophoretic conditions.
Patent
Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification
TL;DR: In this article, a method for the identification of microorganisms is disclosed, comprising first isolating genomic DNA from the suspect microorganism, and then amplification reaction is carried out in such manner as to either amplify the variable sequences interspersed between highly conserved rDNA sequences or to amplify arbitrary genomic regions in conjunction with the variable sequence.
Patent
Improved method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction.
Grosz Ron,Mark Anton Jensen +1 more
TL;DR: In this paper, an improved method for performing nested polymerase chain reaction (PCR) amplification of a targeted piece of DNA was proposed, wherein by controlling the annealing times and concentration of both the outer and the inner set of primers according to the method disclosed, highly specific and efficient amplification was achieved within one reaction vessel without depletion or removal of the outer primers from the reaction mixture vessel.