Showing papers by "Martha Skinner published in 1969"
TL;DR: Whether this molecular structure was consistently present in all preparations, including those freed from the pentagonal unit, was determined to be consistently present.
Abstract: The major component of amyloid is a fibrous protein (1) with a unique organization as seen by high resolution electron microscopy (2). A second component, a pentagonal rod, has been isolated from amyloid deposits and demonstrated to be identical with a circulating a globulia (P-component) (3). This component is distinct from the fibril in its ultrastructure, chemistry, and immunological interactions and constitutes only a minute part of amyloid deposits.In 1960 we obtained an X-ray diffraction powder pattern of an amyloid-rich tissue. In subsequent years improved methods of isolation of amyloid were devised and X-ray diffraction patterns of these more purified preparations were obtained. Results were presented1 that indicated the presence of a cross-β pattern. The present study was carried out on amyloid fibrils isolated by various techniques to determine whether this molecular structure was consistently present in all preparations, including those freed from the pentagonal unit.Meterials and Methods Amyl...
155 citations
TL;DR: Eighteen patients had definite calcium pyrophosphate dihydrate crystal deposition disease (pseudogout) as shown by clinical and roentgenographic findings plus results of synovial fluid analyses.
Abstract: Eighteen patients had definite calcium pyrophosphate dihydrate crystal deposition disease (pseudogout) as shown by clinical and roentgenographic findings plus results of synovial fluid analyses. Seventy-two percent of the patients had diabetes mellitus and 33% had hypertension. Hyperuricemia was an uncommon finding except in association with azotemia and in one patient who also had gout. All patients had typical calcium pyrophosphate crystals in the synovial fluid and one patient also had an unidentified crystal. The character of the synovial fluid appeared to relate to the duration of the acute attack. The fluid was generally of an inflammatory type. Chondrocalcinosis was present roentgenographically in all joints where crystals were found and often in several other joints. Simple therapeutic measures (arthrocenteses) usually relieved the acute articular symptoms.
147 citations