M
Melinda K. Kauffman
Researcher at Washington and Lee University
Publications - 2
Citations - 151
Melinda K. Kauffman is an academic researcher from Washington and Lee University. The author has contributed to research in topics: Fluorescence microscope & Mitochondrial ROS. The author has an hindex of 2, co-authored 2 publications receiving 87 citations.
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Journal ArticleDOI
MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS
Megan E. Kauffman,Melinda K. Kauffman,Kassim Traore,Hong Zhu,Michael A. Trush,Zhenquan Jia,Y. Robert Li +6 more
TL;DR: This ROS Protocols article described the flow cytometric protocol involving the use of various concentrations of MitoSOX (1, 2.5, 5 μM) for detecting mitochondrial ROS in control and mitochondrial DNA-deficient melanoma B16-F10 cells and suggested that 1 μM, rather than the commonly used 5μM, appeared to be the optimal concentration.
Journal ArticleDOI
Fluorescence-Based Assays for Measuring Doxorubicin in Biological Systems.
TL;DR: The use of fluorescence-based techniques, including fluorescence spectrometry, microscopy, and flow cytometry, in measuring and/or detecting doxorubicin in biological systems, including cell lysates and cultured intact cells are characterized for the first time.