The invention provides novel polypeptides having phospholipase activity, including, eg, phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them Industrial methods, eg, oil degumming, and products comprising use of these phospholipases are also provided

Phospholipases, nucleic acids encoding them and methods for making and using them

Disclosed are methods of preparing high-quality polyunsaturated fatty acid-containing lipids from a lipid-containing material that include enzymatic treatment of components of the material and/or pressure disruption of the material. Lipid-containing materials include biomass, such as microorganisms. The invention further includes products containing the lipid compositions, such as dietary supplements, food products, pharmaceutical formulations, humanized animal milk, and infant formula.

Method for producing lipids by liberation from biomass

An oil/fat composition comprising 5 to 99.9 wt.% of a monoglyceride having, as fatty acid constituents thereof, 15 to 90 wt% of an φ 3-unsaturated fatty acid having less than 20 carbon atoms, 1 to 80 wt.% of an φ 9-unsaturated fatty acid and 2 to 50 wt.% of an φ 9-unsaturated fatty acid; and 0.1 to 49.9 wt.% of a diglyceride, wherein a weight ratio of the diglyceride to the monoglyceride is less than 1 and the content of a polyunsaturated fatty acid having at least 4 carbon-to-carbon double bonds is 20% or less in all the fatty acid constituents. The oil/fat composition according to the present invention has excellent processing properties, good taste and excellent lowering action against glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in blood. It is useful not only for pharmaceuticals but also for preventive or remedial foods of feeds effective for hepatic function disturbances or obesity.

Oil/fat composition

A pharmaceutical composition in the form of an emulsion preconcentrate for oral administration and containing a cyclosporin. The pharmaceutical composition has a carrier medium for the cyclosporin that contains a hydrophilic organic solvent; a mixed mono-, di-, and tri-glyceride or a transesterified and polyethoxylated vegetable oil; and a polyoxyethylene-sorbitan-fatty acid ester surfactant. The pharmaceutical composition provides high bioavailability and low inter- and intra-subject variability.

Pharmaceutical compositions containing cyclosporins

Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.

Hydrolases, nucleic acids encoding them and methods for making and using them

An enzymatic transesterification method for preparing a margarine oil having both low trans- acid and low intermediate chain fatty acid content is disclosed. The method includes the steps of providing a transesterification reaction mixture containing a stearic acid source material and an edible liquid vegetable oil, transesterifying the stearic acid source material and the vegetable oil using a 1-, 3-positionally specific lipase, and then finally hydrogenating the fatty acid mixture to provide a recycle stearic acid source material for a recyclic reaction with the vegetable oil. Also described is a counter-current method for preparing a transesterified oil. The method includes the steps of providing a transesterification reaction zone containing a 1-, 3-positionally specific lipase, introducing a vegetable oil into the transesterification zone, introducing a stearic acid source material, conducting a supercritical gas or subcritical liquified gas counter-current fluid, carrying out a transesterification reaction of the triglyceride stream with the stearic acid or stearic acid monoester stream in the reaction zone, withdrawing a transesterified triglyceride margarine oil stream, withdrawing a counter-current fluid phase, hydrogenating the transesterified stearic acid or stearic acid monoester to provide a hydrogenated recycle stearic acid source material, and introducing the hydrogenated recycle stearic acid source material into the reaction zone.

Enzymatic method for preparing transesterified oils

MARGARINE OILS HAVING BOTH LOW TRANS-AND LOW INTERMEDIATE CHAIN SATURATE CONTENT Abstract Transesterified margarine oil products having smooth mouthfeel and broad melting range characteristics which contain very low C8-C16 saturated fatty acids and low trans- unsaturated fatty acid content, as well as enzymatic methods for preparing such margarine oil products.

Margarine oils having both low trans- and low intermediate chain saturate content

Transesterified vegetable oils having a very low saturated fatty acid content and batch, cocurrent and countercurrent enzymatic transesterification methods of preparing such oils are disclosed. The elible fats and oils are a rich source of energy in the diet and are important in the synthesis of membranes and other cell components.

Low-saturate edible oils and transesterification methods for production thereof

Huiles vegetales transesterifiees presentant une faible teneur en acides gras satures, et leurs procedes de preparation en lots par transesterification enzymatique a co-courant et a contre-courant. Les matieres grasses et les huiles comestibles constituent une source riche en energie dans l'alimentation, et sont importantes dans la synthese de membranes et d'autres constituants cellulaires.

Michael T. Dueber

Papers

Enzymatic method for preparing transesterified oils

Margarine oils having both low trans- and low intermediate chain saturate content

Low-saturate edible oils and transesterification methods for production thereof

Huiles comestibles faiblement satures et leurs procedes de production par transesterification