Showing papers by "Miguel G. Guerrero published in 2009"
TL;DR: The magnitude of the experimental values demonstrate that astaxanthin can be efficiently produced outdoors in continuous mode through a precise dosage of the specific nitrate input, taking also into consideration the average irradiance inside the culture.
Abstract: The feasibility of a one-step method for the continuous production of astaxanthin by the microalga Haematococcus pluvialis has been verified outdoors. To this end, influence of dilution rate, nitrate concentration in the feed medium, and irradiance on the performance of continuous cultures of H. pluvialis was firstly analyzed indoors in bubble column reactors under daylight cycles, and then outdoors, using a tubular photobioreactor. At the laboratory scale, the behavior of the cultures agreed with that previously recorded in continuous illumination experiences, and attested that the major factors determining biomass and astaxanthin productivity were average irradiance and specific nitrate supply. The rate of astaxanthin accumulation was proportional to the average irradiance inside the culture, provided that a nitrate limiting situation had been established. The accumulation of astaxanthin under daylight cycles was maximal for a specific nitrate input of 0.5 mmol/g day. The recorded performance has been modeled on the basis of previously developed equations, and the validity of the model checked under outdoor conditions. Productivity values for biomass and astaxanthin of 0.7 g/L day and 8.0 mg/L day respectively, were obtained in a pilot scale tubular photobioreactor operating under continuous conditions outdoors. The magnitude of the experimental values, which matched those simulated from the obtained model, demonstrate that astaxanthin can be efficiently produced outdoors in continuous mode through a precise dosage of the specific nitrate input, taking also into consideration the average irradiance inside the culture. Biotechnol. Bioeng. 2009;102: 651–657. © 2008 Wiley Periodicals, Inc.
115 citations
TL;DR: These findings confirm and extend the association of this gene with MS and reveal a genetic heterogeneity of the associated polymorphisms and risk alleles between MS and T1D suggesting different immunopathological roles of IL2RA in these two diseases.
Abstract: Financial support for the study was provided by the Ministerio de Educacion y Ciencia (grants PN-SAF2006-02023 and TIN2007-67418-C03-03) and Junta de Andalucia (P07-CVI-02551) to A. Alcina and Servicio Andaluz de Salud de la Junta de Andalucia (grant PI0168/2007) to F. Matesanz. Maria Fedetz is a holder of a fellowship from Fundacion IMABIS. Dorothy Ndagire is a holder of AECI-Ministerio de Asuntos Exteriores fellowship.
81 citations
TL;DR: In vitro DNA-protein interaction analysis reveals that the response regulator Rre1 binds specifically to the promoter region of the adhA gene, which encodes a member of the medium-chain alcohol dehydrogenase/reductase family.
Abstract: The slr1192 (adhA) gene from Synechocystis sp. strain PCC 6803 encodes a member of the medium-chain alcohol dehydrogenase/reductase family. The gene product AdhA exhibits NADP-dependent alcohol dehydrogenase activity, acting on a broad variety of aromatic and aliphatic primary alcohols and aldehydes but not on secondary alcohols or ketones. It exhibits superior catalytic efficiency for aldehyde reduction compared to that for alcohol oxidation. The enzyme is a cytosolic protein present in photoautotrophically grown Synechocystis cells. The expression of AdhA is enhanced upon the exposure of cells to different environmental stresses, although it is not essential for survival even under such stress conditions. The induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system, as it is severely impaired in mutant strains lacking either the histidine kinase Hik34 or the response regulator Rre1. In vitro DNA-protein interaction analysis reveals that the response regulator Rre1 binds specifically to the promoter region of the adhA gene.
73 citations
TL;DR: The results did not show major influence of TENR-IL2-IL21 locus on susceptibility or disease progression in MS, but could not exclude completely the effect in MS for this region.
Abstract: This work was supported by grants PN-SAF2006-02023 and Junta de Andaluci´a P07-CVI-02551 to AA and Servicio Andaluz de Salud PI0168/2007 to FM, and MF is a holder of a fellowship from The Mediterranean Institute for the Advance Biotechnology and Health Research Foundation (IMABIS). DN is a holder of the Spanish Agency for International Cooperation (AECI) – Ministerio de Asuntos Exteriores fellowship.
24 citations