The origin of plant cell and tissue culture can be found in a treatise published during the mid-18th century, entitled La Physique des Arbes, that describes the formation of callus tissue following the for mation of a ring of cortex from elm trees. Over the next two centuries, the discovery of plant growth hormones, in particular auxins and cytokinins, and detailed analyses on the nutritional requirements of plants, led to the formulation of media that could maintain actively dividing cultures derived from gymnosperms, and both dicotyledon ous and monocotyledonous angiosperms. However, much of the prog ress and technological development in the in vitro propagation of plant cells, tissues, and organs has occurred during the last 25 years. Recently, plant tissue culture techniques have been used as basic tools in the rapidly expanding field of plant biotechnology for the development and clonal propagation of new and/or improved plant varieties. Plant tissue culture is used for the micropropagation of commercially valuable cultivars that include ornamentals, oil palm, Glycyrrhiza, Pyrethrum, pine, Eucalyptus, sugar cane, and potatoes. Cultured plant tissue is also used for the selection of cells and, ul timately, the regeneration of plants that are tolerant to physical stresses such as pathogens, drought, and temperature extremes, and to chemical stress agents such as salinity, herbicides, proteins, and pyrethrins. In addition, new plants have been produced by the fusion of protoplasts prepared from cultured cells of different species in cluding sunflower and french bean, tomato and potato, and various cultivars of Datura. Finally, bacterial vectors and various mechanical methods have been used to introduce foreign genes into cultured plant tissues. Genetic transformation can result in profound changes in the phenotype and/or biochemical profile of the regenerated trans genic plants that are not characteristic of the wild type. An impressive variety of technologies in tissue culture, genetic manipulation, and molecular biology have been developed for nu merous plant species. Many of these techniques, sometimes referred to as plant biotechnology, have been extensively summarized and compiled in a well-balanced collection of easy-to-follow protocols presented in three separate, but complimentary, volumes. Plant Cell, Tissue and Organ Culture consists of 22 chapters (with 86 figures) and 5 appendices. The chapters cover critical aspects of (a) the es sential requirements for the operation of a plant tissue culture lab oratory; (b) the basic procedures of micropropagation, regeneration, and somatic embryogenesis; (c) some specific applications of organ culture systems such as embryo rescue and culture, and anther and microspore culture for haploid and double haploid production; (d) elementary transformation technology; and (e) useful microtechnique and analytical protocols specifically adapted to cultured tissues and cells. The appendices provide a convenient summary of media for mulations and commercial suppliers for the materials described in the text.

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Plant Cell, Tissue and Organ Culture

Background: Current understanding of the spatial epidemiology and geographical distribution of Plasmodium vivax is far less developed than that for P. falciparum, representing a barrier to rational strategies for control and elimination. Here we present the first systematic effort to map the global endemicity of this hitherto neglected parasite. Methodology and Findings: We first updated to the year 2010 our earlier estimate of the geographical limits of P. vivax transmission. Within areas of stable transmission, an assembly of 9,970 geopositioned P. vivax parasite rate (PvPR) surveys collected from 1985 to 2010 were used with a spatiotemporal Bayesian model-based geostatistical approach to estimate endemicity age-standardised to the 1–99 year age range (PvPR1–99) within every 565 km resolution grid square. The model incorporated data on Duffy negative phenotype frequency to suppress endemicity predictions, particularly in Africa. Endemicity was predicted within a relatively narrow range throughout the endemic world, with the point estimate rarely exceeding 7% PvPR1–99. The Americas contributed 22% of the global area at risk of P. vivax transmission, but high endemic areas were generally sparsely populated and the region contributed only 6% of the 2.5 billion people at risk (PAR) globally. In Africa, Duffy negativity meant stable transmission was constrained to Madagascar and parts of the Horn, contributing 3.5% of global PAR. Central Asia was home to 82% of global PAR with important high endemic areas coinciding with dense populations particularly in India and Myanmar. South East Asia contained areas of the highest endemicity in Indonesia and Papua New Guinea and contributed 9% of global PAR.

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A long neglected world malaria map: Plasmodium vivax endemicity in 2010.

Background: A research priority for Plasmodium vivax malaria is to improve our understanding of the spatial distribution of risk and its relationship with the burden of P. vivax disease in human populations. The aim of the research outlined in this article is to provide a contemporary evidence-based map of the global spatial extent of P. vivax malaria, together with estimates of the human population at risk (PAR) of any level of transmission in 2009. Methodology: The most recent P. vivax case-reporting data that could be obtained for all malaria endemic countries were used to classify risk into three classes: malaria free, unstable (,0.1 case per 1,000 people per annum (p.a.)) and stable ($0.1 case per 1,000 p.a.) P. vivax malaria transmission. Risk areas were further constrained using temperature and aridity data based upon their relationship with parasite and vector bionomics. Medical intelligence was used to refine the spatial extent of risk in specific areas where transmission was reported to be absent (e.g., large urban areas and malaria-free islands). The PAR under each level of transmission was then derived by combining the categorical risk map with a high resolution population surface adjusted to 2009. The exclusion of large Duffy negative populations in Africa from the PAR totals was achieved using independent modelling of the gene frequency of this genetic trait. It was estimated that 2.85 billion people were exposed to some risk of P. vivax transmission in 2009, with 57.1% of them living in areas of unstable transmission. The vast majority (2.59 billion, 91.0%) were located in Central and South East (CSE) Asia, whilst the remainder were located in America (0.16 billion, 5.5%) and in the Africa+ region (0.10 billion, 3.5%). Despite evidence of ubiquitous risk of P. vivax infection in Africa, the very high prevalence of Duffy negativity throughout Central and West Africa reduced the PAR estimates substantially. Conclusions: After more than a century of development and control, P. vivax remains more widely distributed than P. falciparum and is a potential cause of morbidity and mortality amongst the 2.85 billion people living at risk of infection, the majority of whom are in the tropical belt of CSE Asia. The probability of infection is reduced massively across Africa by the frequency of the Duffy negative trait, but transmission does occur on the continent and is a concern for Duffy positive locals and travellers. The final map provides the spatial limits on which the endemicity of P. vivax transmission can be mapped to support future cartographic-based burden estimations.

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The international limits and population at risk of Plasmodium vivax transmission in 2009

Allelopathy and Exotic Plant Invasion: From Molecules and Genes to Species Interactions

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Field And Laboratory Methods For General Ecology

The present study was carried out to isolate fungi associated with seeds of seven cultivars of sunflower by using agar and blotter paper methods. A total of 13 phytopathogenic fungal species including Alternaria alternata and A. helianthi, Aspergillus flavus, A. fumigatus and A. niger, Curvularia lunata, Drechslera tetramera, Fusarium solani and F. moniliforme, Macrophomina phaseolina, Mucor mucedo, Penicillium and Rhizopus spp. were identified. The isolated fungi were found to reduce seed germination by 10-20% and seedling mortality by 10-12%. Two systemic fungicides viz., Topsin and Bayleton were found to be significantly effective in the elimination of seed-borne fungi. Among the plant material, best antifungal activity was achieved by extracts of Azadirachta indica (Neem), and Allium sativum (garlic) at the concentration of 0.015%.

Mycoflora associated with seeds of different sunflower cultivars and its management.

Cladophora is a group of macroscopic green algae with over 183 species. This genus is rich in phytochemical compounds that can be exploited for sustaining both human and animal health. Owing to the variety of secondary metabolites, species of this group possess antioxidant anti-diabetic, anti-hypertensive, anti-parasitic, anti-microbial, anti-cancer and cytotoxicity activities. Although considerable research has been conducted previously for isolation and identification of pharmaceutically important compounds from Cladophora very few compounds are commercialized. This review encompasses the nutraceutical, pharmaceutical, health and research potential of different bioactive compounds present in Cladophora. Aquaculture techniques used for high biomass production to fulfill commercial demand are also discussed.

Pharmaceutical aptitude of Cladophora: A comprehensive review

https://iopscience.iop.org/article/10.1088/2053-1591/ac1de3/pdf

Application of green synthesized silver nanoparticles in cancer treatment—a critical review

The present study was aimed to record the flora of Khanpur Dam, Khyber Pakhtunkhwa, Pakistan. For this purpose, the whole area was surveyed during 2009 to 2010 for the collection of plant specimens. A total of 221 plant species of 169 genera and 66 families were recorded from the study area, including two ferns, one gymnosperm, 39 monocots and 179 dicots. Poaceae was found as the most dominant family in the flora of the project area that contributed 33 species (14. 86%), followed by Asteraceae 26 spp. (11.71%), Fabaceae 13 spp. (5.86%), Amaranthaceae & Lamiaceae 9 spp. each (4.05% each), Euphorbiaceae & Solanaceae 8 spp. (3.60% each), Polygonaceae 7 spp. (3.15%) and Brassicaceae 6 spp. (2.70%), while rest of the families shared 1-5 species. It was observed that most of the taxa were perennials (49.32%) followed by annuals (47.51%) and biennial (3.17%). Herbs were dominating fraction in the floristic composition that contributed 57.47%, followed by shrubs (14.03%), grasses (10.86%) and trees (9.50%), whereas rest of 5 categories were in the range of 3.17-0.45%. Therophytes were the most abundant life form that constituted 42.53% of the total flora, followed by phanerophytes (27.15%), hemicryptophytes (18.55%), chamaephytes (7.24%) and cryptophytes (4.52%). Two species viz., Alternanthera paronychioides and Boerhavia diandra are reported for the first time from Khyber Pakhtunkhwa province.

Phytodiversity and plant life of khanpur dam, khyber pakhtunkhwa, pakistan

A point survey of malaria in Karachi revealed a concentration of malaria cases in the periurban perimeter and marginal areas where immigrating groups, including Afghan refugees and Biharis from Bangladesh are concentrated. Populations of Anopheles stephensi, Karachi's main vector, were low, and were feeding chiefly on buffaloes which are also concentrated along the periurban perimeter. Maintenance of malaria transmission in periurban zones may depend on immigration of susceptibles, bovid hosts which help maintain zoophilic vector populations, and mosquito breeding sites in water tanks or buffalo hoofprints. The vector's switch to man-biting behaviour may occur either due to a 'spillover' effect from buffalo corrals to surrounding houses, or when buffaloes become less available for biting. Four types of chromosome inversions were found in the Karachi An. stephensi; as this species is not a vector in similar habitats in Lahore and Pondicherry where such inversions are not prevalent, it is likely that its status as a vector is related to chromosomal polymorphism influencing behaviour.

Mubashrah Munir

Papers

Mycoflora associated with seeds of different sunflower cultivars and its management.

Pharmaceutical aptitude of Cladophora: A comprehensive review

Application of green synthesized silver nanoparticles in cancer treatment—a critical review

Phytodiversity and plant life of khanpur dam, khyber pakhtunkhwa, pakistan

A point survey of periurban and urban malaria in Karachi.