The use of antibiotics may accelerate the development of antibiotic resistance genes (ARGs) and bacteria which shade health risks to humans and animals. The emerging of ARGs in the water environment is becoming an increasing worldwide concern. Hundreds of various ARGs encoding resistance to a broad range of antibiotics have been found in microorganisms distributed not only in hospital wastewaters and animal production wastewaters, but also in sewage, wastewater treatment plants, surface water, groundwater, and even in drinking water. This review summarizes recently published information on the types, distributions, and horizontal transfer of ARGs in various aquatic environments, as well as the molecular methods used to detect environmental ARGs, including specific and multiplex PCR (polymerase chain reaction), real-time PCR, DNA sequencing, and hybridization based techniques.

Antibiotic resistance genes in water environment.

OP/nerve agents are still considered as important chemicals acting on living organisms and are widely used. They are characterized according to their action as compounds influencing cholinergic nerve transmission via inhibition of AChE. Modeling of this action and extrapolation of experimental data from animals to humans is more possible for highly toxic agents than for the OP. The symptoms of intoxication comprise nicotinic, muscarinic, and central symptoms; for some OP/nerve agents, a delayed neurotoxicity is observed. Cholinesterases (AChE and BuChE) are characterized as the main enzymes involved in the toxic effect of these compounds, including molecular forms. The activity of both enzymes (and molecular forms) is influenced by inhibitors (reversible, irreversible, and allosteric) and other factors, such as pathological states. There are different methods for cholinesterase determination; however, the most frequent is the method based on the hydrolysis of thiocholine esters and subsequent detection of free SH-group of the released thiocholine. The diagnosis of OP/nerve agent poisoning is based on anamnesis, the clinical status of the intoxicated organism, and on cholinesterase determination in the blood. For nerve agent intoxication, AChE in the red blood cell is more diagnostically important than BuChE activity in the plasma. This enzyme is a good diagnostic marker for intoxication with OP pesticides. Some other biochemical examinations are recommended, especially arterial blood gas, blood pH, minerals, and some other specialized parameters usually not available in all clinical laboratories. These special examinations are important for prognosis of the intoxication, for effective treatment, and for retrospective analysis of the agent used for exposure. Some principles of prophylaxis against OP/nerve agent poisoning comprising the administration of reversible cholinesterase inhibitors such as pyridostigmine (alone or in combination with other drugs), scavengers such as preparations of cholinesterases, some therapeutic drugs, and possible combinations are given. Basic principles of the treatment of nerve agent OP poisoning are described. They are based on the administration of anticholinergics (mostly atropine but some other anticholinergics can be recommended) as a symptomatic treatment, cholinesterase reactivators as a causal treatment (different types but without a universal reactivator against all OP/nerve agents) as the first aid and medical treatment, and anticonvulsants, preferably diazepam though some other effective benzodiazepines are available. New drugs for the treatment are under experimental study based on new approaches to the mechanism of action. Future trends in the complex research of these compounds, which is important not only for the treatment of intoxication but also for the quantitative and qualitative increase of our knowledge of toxicology, neurochemistry, neuropharmacology, clinical biochemistry, and analytical chemistry in general, are characterized.

Organophosphates⧸Nerve Agent Poisoning: Mechanism of Action, Diagnosis, Prophylaxis, And Treatment

Summary: Blastocystis is an unusual enteric protozoan parasite of humans and many animals. It has a worldwide distribution and is often the most commonly isolated organism in parasitological surveys. The parasite has been described since the early 1900s, but only in the last decade or so have there been significant advances in our understanding of Blastocystis biology. However, the pleomorphic nature of the parasite and the lack of standardization in techniques have led to confusion and, in some cases, misinterpretation of data. This has hindered laboratory diagnosis and efforts to understand its mode of reproduction, life cycle, prevalence, and pathogenesis. Accumulating epidemiological, in vivo, and in vitro data strongly suggest that Blastocystis is a pathogen. Many genotypes exist in nature, and recent observations indicate that humans are, in reality, hosts to numerous zoonotic genotypes. Such genetic diversity has led to a suggestion that previously conflicting observations on the pathogenesis of Blastocystis are due to pathogenic and nonpathogenic genotypes. Recent epidemiological, animal infection, and in vitro host-Blastocystis interaction studies suggest that this may indeed be the case. This review focuses on such recent advances and also provides updates on laboratory and clinical aspects of Blastocystis spp.

New insights on classification, identification, and clinical relevance of Blastocystis spp.

The oral health surveys basic methods that we provide for you will be ultimate to give preference. This reading book is your chosen book to accompany you when in your free time, in your lonely. This kind of book can help you to heal the lonely and get or add the inspirations to be more inoperative. Yeah, book as the widow of the world can be very inspiring manners. As here, this book is also created by an inspiring author that can make influences of you to do more.

Oral Health Surveys: Basic Methods

Chemical composition and antimicrobial activity of the essential oils of Chrysanthemum indicum

Purpose To investigate the effect of sandblasting powder particles on microtensile bond strength (μTBS) of dual-cure adhesive cement to CAD/CAM blocks. Materials and methods CAD/CAM blocks (Cerasmart, VITA, and LAVA) were cut in slabs and divided into groups: group 1, no sandblasting; group 2, sandblasted with 27-μm Al2 O3 ; group 3, sandblasted with 30-μm CoJet; group 4, sandblasted with 50-μm Al2 O3 . After sandblasting, all specimens were silanized and luted using dual-cure adhesive cement (G-CEM LinkForce). After 24 hours, bonded specimens were cut into 1 ± 0.2 mm2 sticks, and μTBS values were obtained (N = 30). Additionally, 132 CAD/CAM block sections were prepared for surface roughness testing and scanning electron microscopy (SEM) evaluations. Results were analyzed using Kruskal-Wallis One-way ANOVA and Dunn's Post Hoc Test (p Results Group 1 exhibited significantly lower μTBS than the other groups (p 0.05). For LAVA, μTBS values of specimens that were sandblasted with 50-μm Al2 O3 powder were significantly higher than 30-μm-SiO2 and 27-μm Al2 O3 (p Conclusions The sand particles investigated (27-μm Al2 O3 , 30-μm SiO2 , or 50-μm Al2 O3 ) did not significantly affect μTBS results of CAD/CAM blocks for Cerasmart and VITA, although the results changed significantly for LAVA. The ideal bond protocol for CAD/CAM blocks is specific to the material used.

Microtensile Bond Strength of CAD/CAM Resin Blocks to Dual‐Cure Adhesive Cement: The Effect of Different Sandblasting Procedures

Purpose To evaluate the effect of prolonged sandblasting on the bond durability of dual-cure adhesive resin cement to computer-aided design and computer-aided manufacturing (CAD/CAM) restoratives. Materials and methods Nano-ceramic LAVA Ultimate and hybrid-ceramic VITA Enamic CAD/CAM blocks were used for this study. Each CAD/CAM block was sectioned into slabs of 4-mm thickness for the microtensile test (µTBS) test and 2-mm thickness for the surface roughness test. Three groups were created according to the sandblasting protocols; group 1: specimens were sandblasted for 15 seconds, group 2: specimens were sandblasted for 30 seconds, and group 3: specimens were sandblasted for 60 seconds. After sandblasting, all specimens were luted using RelyX Ultimate Clicker. Half the specimens were subjected to µTBS tests at 24 hours, and the other half were subjected to tests after 5000 thermocycles. Additionally, a total of 96 CAD/CAM block sections were prepared for surface roughness tests and scanning electron microscopy (SEM) evaluations. The Mann-Whitney U test, Kruskal-Wallis one-way analysis of variance, and Dunn's post hoc test were used to compare continuous variables among the groups. Results At baseline, group 1, group 2, and group 3 exhibited statistically similar µTBS results for LAVA. However, group 3 had significantly lower µTBS values than groups 1 and 2 for VITA. After 5000 thermocycles, µTBS values significantly decreased for each block (P Conclusion It is important to perform controlled sandblasting because it may affect bond strength results. Sixty seconds of sandblasting disturbs the initial µTBS values and the stability of adhesion of CAD/CAM restoratives to dual-cure adhesive resin cement for VITA Enamic.

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The effect of sandblasting duration on the bond durability of dual-cure adhesive cement to CAD/CAM resin restoratives.

Eucalyptus trees are widespread in subtropical parts of Turkey that have alkaline environments due to the soil structure of Taurus Mountains. In this study, the existence of Cryptococcus neoformans in eucalyptus trees in the South Aegean and Mediterranean Regions of Anatolia, Turkey, was screened between March 1998 and September 2002. Only one strain of Cryptococcus neoformans var. grubii (Serotype A) was isolated from 1175 eucalyptus samples including debris and flowers in culture by Guizotia abyssinica agar. The environmental niche of the isolate was Eucalyptus camaldulensis Dehn in the Gokova Region, in the western part of the Taurus Mountains. In this study, the existence of Cryptococcus neoformans was shown in the eucalyptus flora of Turkey despite the alkaline soil condition.

The first isolation of Cryptococcus neoformans from Eucalyptus trees in South Aegean and Mediterranean Regions of Anatolia in Turkey despite Taurus Mountains alkalinity.

– Groundwater Level Predıctıon Usıng Artıfıcıal Neural Network and M5 Tree Models. Most of the fresh water resources in our world consist of underground water reserves. Estimation of fluctuations of groundwater level (GWL) is very important in the management of water resources. In this study, groundwater level (GWL) was investigated using artificial neural networks (ANN), M5tree (M5T) approaches in Reyhanlı region in Turkey. Total 196 data from 2000-2015 taken from 1 observation station belonging to Reyhanlı sub-basin located in Asi basin were used in the study. Using the monthly average precipatation and temperature, the change in GWL is modeled by artificial neural networks (ANN), M5tree (M5T) approaches. The results showed that (ANN) and M5tree (M5T) models were found to be very close to each other.

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Groundwater Level Prediction Using Artificial Neural Network and M5 Tree Models

In this study, a high sensitive metamaterial sensor has been designed to detect quality of water for water treatment centre. The water samples have been obtained and electrical properties have been measured in microwave range to design the proposed sensor. These electrical properties have been assigned in microwave simulator. Water quality has been investigated by using parametric study and genetic algorithm approach to realize high sensitivity in terms of resonance frequency shift. Around 130 MHz frequency shift has been observed between the water samples. This shift is sufficient to detect instantaneously to the quality of water. This is the first study that metamaterial approach has been used to detect water quality in literature.

Mustafa Demirci

Papers

Microtensile Bond Strength of CAD/CAM Resin Blocks to Dual‐Cure Adhesive Cement: The Effect of Different Sandblasting Procedures

The effect of sandblasting duration on the bond durability of dual-cure adhesive cement to CAD/CAM resin restoratives.

The first isolation of Cryptococcus neoformans from Eucalyptus trees in South Aegean and Mediterranean Regions of Anatolia in Turkey despite Taurus Mountains alkalinity.

Groundwater Level Prediction Using Artificial Neural Network and M5 Tree Models

High Sensitive Metamaterial Sensor for Water Treatment Centres