Application of biocatalysis and biotransformations to the synthesis of pharmaceuticals

Preparation and characterization of Pseudomonas putida esterase immobilized on magnetic nanoparticles

The configuration of a feedstock material is controlled by bringing it into contact with at least a first gas moving against it at a location with an area and thickness of the feedstock liquid that forms drops or fibers of a selected size. In one embodiment, drops of agricultural input materials are formed for spraying on agricultural fields. In another embodiment, nanofibers of materials such as chitosan or metals are formed. In another embodiment seeds are planted with gel. In another embodiment particles carrying desired agricultural inputs with modified release characteristics are delivered.

Systems for the control and use of fluids and particles

A novel lactonohydrolase, an enzyme that catalyzes the hydrolysis of 3,4-dihydrocoumarin, was purified 375-fold to apparent homogeneity, with a 22.7% overall recovery, from Acinetobacter calcoaceticus F46, which was isolated as a fluorene-assimilating micro-organism. The molecular mass of the native enzyme, as estimated by high-performance gel-permeation chromatography, is 56 kDa, and the subunit molecular mass is 30 kDa. The enzyme specifically hydrolyzes 3,4-dihydrocoumarin, and the Km and Vmax for 3,4-dihydrocoumarin are 0.806 mm and 4760 U·mg−1, respectively. The N-terminal and internal amino acid sequences of the enzyme show high similarity to those of bacterial non-heme haloperoxidases. The enzyme exhibits brominating activity with monochlorodimedon in the presence of H2O2 and 3,4-dihydrocoumarin or an organic acid, such as acetate and n-butyrate.

3,4‐Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46

This invention relates to antimicrobial polyester-containing articles and methodology for the preparation of antimicrobial polyester-containing articles utilizing chitosan and chitosan-metal complexes as the antimicrobial agent.

Antimicrobial polyester-containing articles and process for their preparation

Microorganisms producing D-β-acetylthioisobutyric acid from methyl D-β-acetylthioisobutyrate were screened from stock cultures. The D-β-acetylthioisobutyric acid-producing ability was found in 15 strains belonging to the genera Pseudomonas, Agrobacterium, Enterobacter, Cellulomonas, Rhodococcus, Brevibacterium, and Torulopsis. A strain of Pseudomonas fluorescens, IFO 3081, was selected as the best microorganism. The cells having activity (558 units/g of dry cells) could be easily prepared by cultivation at 25°C at pH 6.6 for 24 hr in a glucose-containing medium. The D-form of methyl DL-β-acetylthioisobutyrate was selectively hydrolyzed with the cells so that D-β-acetylthioisobutyric acid (97.2% enantiomeric excess) was produced in a high yield.

Screening of Microorganisms Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate

The present invention is directed to chitosan-containing acrylic fibers having a total chitosan content of 0.05 to 2% by weight and an extractable chitosan content of not less than 0.03% by weight to less than the total chitosan content. The antimicrobial activity of the chitosan-containing acrylic fibers of the present invention can persist for a long period of time and is not deteriorated even when subjected to posttreatments, such as dyeing and bleaching of fibers, and treatments in usual service environments of fiber products, such as washing and ironing.

Chitosan-containing acrylic fibers and process for preparing the same

The process conditions concerned with the production of DL-β-acetylthioisobutyric acid were investigated. Methyl DL-β-acetylthioisobutyrate as an enzyme substrate was produced from methyl methacrylate in 96.6% yield by allowing 1.0 mol of methyl methacrylate to react with 1.6 mol of thioacetic acid at 80oC for 6 h. Through the study of hydrolytic reaction of methyl DL-β-acetylthioisobutyrate with the cells of Pseudomonas putida MR-2068, it was found that D-β-acetylthioisobutyric acid having more than 98% (e.e.) optical purity was obtained when the reaction was done below pH 7.0 and below 50oC. For example, 10% (w/v) methyl DL-β-acetylthioisobutyrate in the reaction mixture was asymmetrically hydrolyzed by 0.2% (w/v) cells at 45oC at pH 7.0 for 18h to give DAT having 98.2% (e.e.) optical purity in 49.7% yield. To prevent the decompositon and racemization of D-β-acetylthioisobutyric acid in the purification process, the use of a thin-film distillation apparatus for a continuous distillation was proposed to ...

Process Conditions for Production of D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate with the Cells of Pseudomonas putida MR-2068

Process of making chitosan-containing acrylic fibers

Disclosed is a process for producing dental polymeric shaped articles resistant to the adhesion of plaque which comprises the steps of shaping and curing a dental composition containing a monomer having at least one (meth)acryloyloxy group, and bringing the cured product into contact with a halogen or a halogen compound. Also disclosed is a dental composition comprising a monomer having at least one (meth)acryloyloxy group and a polymerization initiator, which is characterized by having added thereto at least one of a halogen and a halogen compound. When cured, this dental composition produces dental polymeric shaped articles resistant to the adhesion of plaque.

Naoto Ohsuga

Papers

Screening of Microorganisms Producing D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate

Chitosan-containing acrylic fibers and process for preparing the same

Process Conditions for Production of D-β-Acetylthioisobutyric Acid from Methyl DL-β-Acetylthioisobutyrate with the Cells of Pseudomonas putida MR-2068

Process of making chitosan-containing acrylic fibers

Dental composition and process for producing dental polymeric shaped articles