N
Nicola Leyland
Researcher at John Innes Centre
Publications - 4
Citations - 2372
Nicola Leyland is an academic researcher from John Innes Centre. The author has contributed to research in topics: GPX1 & Glutathione. The author has an hindex of 4, co-authored 4 publications receiving 2240 citations. Previous affiliations of Nicola Leyland include Norwich Research Park.
Papers
More filters
Journal ArticleDOI
pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation.
TL;DR: The pGreen plasmid system allows any arrangement of selectable marker and reporter gene at the right and left T-DNA borders without compromising the choice of restriction sites for cloning, since the pGreen cloning sites are based on the well-known pBluescript general vector plasmids.
Journal ArticleDOI
Evidence for a Direct Link between Glutathione Biosynthesis and Stress Defense Gene Expression in Arabidopsis
Louise Ball,Gian Paolo Accotto,Ulrike Bechtold,Gary Creissen,Dietmar Funck,Ana Jiménez,Baldeep Kular,Nicola Leyland,Jaime Mejía-Carranza,Helen Reynolds,Stanislaw Karpinski,Philip M. Mullineaux +11 more
TL;DR: The mutant regulator of APX2 1-1 (rax1-1) was identified in Arabidopsis thaliana that constitutively expressed normally photooxidative stress-inducible ASCORBATE PEROXIDASE2 (APX2) and had ≥50% lowered foliar glutathione levels, suggesting that changes in glutATHione metabolism may be one means of integrating the function of several signaling pathways.
Journal ArticleDOI
Elevated Glutathione Biosynthetic Capacity in the Chloroplasts of Transgenic Tobacco Plants Paradoxically Causes Increased Oxidative Stress
Gary Creissen,John L. Firmin,Michael J. Fryer,Baldeep Kular,Nicola Leyland,Helen Reynolds,G. M. Pastori,Florence A. M. Wellburn,Neil R. Baker,Alan R. Wellburn,Philip M. Mullineaux +10 more
TL;DR: It is suggested that γ-ECS–transformed plants suffered continuous oxidative damage caused by a failure of the redox-sensing process in the chloroplast.
Book ChapterDOI
Barley transformation using Agrobacterium-mediated techniques.
Wendy Harwood,Joanne G. Bartlett,Sílvia C Alves,Matthew Perry,Mark A. Smedley,Nicola Leyland,John W. Snape +6 more
TL;DR: This chapter describes a simple system for the transformation of barley based on the infection of immature embryos with Agrobacterium tumefaciens followed by the selection of transgenic tissue on media containing the antibiotic hygromycin.