Showing papers by "Nicoletta Ronda published in 1999"
TL;DR: Evidence is gathered demonstrating that natural AECA constitute a strictly controlled autoantibody pool, interact with living endothelial cells and take part in the regulation of endothelial function, through direct anti-inflammatory effects.
36 citations
TL;DR: The device described in the chapter is simple and inexpensive but fulfills all the described criteria and has been verified on a well-known model of receptor-ligand internalization, that of insulin and insulin receptor.
Abstract: Publisher Summary The chapter presents a discussion on receptor-ligand internalization. Currently, confocal imaging has provided new insights in the observation of fluorescent specimens. The virtual absence of out-of-focus blurring allows a much better definition of probe localization at the subcellular level together with the possibility of exploiting the three-dimensional (3-D) reconstruction capability of most confocal systems. A self-constructed flow chamber to an inverted confocal scanning laser microscope that allows long-term observation of adherent cells, under controlled micro-environmental conditions have been paired. This method provides images of intact, nonfixed cells and allows one to change culture conditions and to observe living cell responses directly or to perform two-step staining to identify subcellular structures involved in the observed processes. The reliability of this procedure has been verified on a well-known model of receptor-ligand internalization, that of insulin and insulin receptor. The same technique has also been applied to studying the interactions between natural human antibodies, circulating in healthy subjects, and living human endothelial cells, fibroblasts, and proximal tubular epithelial cells. The device described in the chapter is simple and inexpensive but fulfills all the described criteria.
17 citations