Streptozotocin-induced diabetes rsulted in a decrease in the cathepsin D activity (free and total) in rat liver, kidney, brain and heart with a concomitant increase in tissue protein content and amino acids pool size. Treatment with insulin brought about the restoration of the cathepsin D activity to normal or greater than normal levels; tissue protein content and amino acids pool size also returned to normal values.

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Insulin-dependent changes in lysosomal cathepsin D activity in rat liver, kidney, brain and heart

Synthesis, antioxidant and cathepsin D inhibition activity of quaternary ammonium chitosan derivatives.

The effect of thyroidectomy and subsequent treatment with tri-iodothyronine (T3), as well as that of thyrotoxicosis, was examined on cathepsin D activity in the rat liver, kidney and brain. Thyrotoxicosis resulted in a generalized increase in the enzyme activity in the 3 tissues; the effect of other thyroidal states was diverse and tissue-specific.

Thyroid hormones and cathepsin D activity in the rat liver, kidney and brain.

Some of the biochemical indices relevant to the "free radical theory" of aging have been assessed in mice subjected to chronic low-dose whole-body irradiation Radiation exposure results in enhanced accumulation of the lipofuscins in brain, heart, and intestine In these animals, the degree of lipoperoxidation in liver was greatly increased, as were the free activities of acid phosphatase and cathespin, indicating damage to lysosomal membranes The activity of SOD in brain and liver 20,000g post-mitochondrial supernatants was lower in the irradiated mice All these changes arising from chronic whole-body irradiation are similar to those observed during aging and are effectively prevented by dietary supplementation with BHT These observations lend considerable support to the "free radical theory" of aging

Effects of chronic irradiation on age-related biochemical changes in mice.

The lysosomal marker enzymes, arylsulfatase and acid phosphatase, in a tropical burrowing arcid clam Anadara granosa L. have been found to exhibit seasonal variations. The activity of both enzymes decreased with increase in ambient temperature and fell with increase in salinity. Lysosomal latency for these enzymes, however, was not significantly affected by environmental parameters, including salinity, temperature, nutritional status, breeding season, etc. The physico-chemical characteristics of the habitat were found to induce reversible changes in the lysosomal latency and enzyme activity. Exposure to elevated temperature (ΔT=7° to 8°C), towards the upper limit of its physiological tolerance, was found to labilize the lysosomes. The biochemical methods optimized for the demonstration of lysosomal stability under exposure to environmental stressors could also be extended to quantify the impact of various pollutants, including heavy metals, radionuclides, etc. at the subcellular level.

Ninjoor

Papers

Influence of vitamin A acetate, alcohol and their radiolytic products on the in vitro labilization of lysosomes and tissue autolysis.

Differential release of latent lysosomal hydrolases in muscle of Tilapia mossambica by whole body gamma radiation.

Alterations in liver lysosomes induced by whole-body x-irradiation of rats.

Nitrite-induced inhibition of purified fractions of chicken muscle cathepsin D.

ビタミンA酢酸エステル,ビタミンAアルコール,これらの照射分解産物のin vitroでのリソゾームの不活性化への影響,および組織の自己分解