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Nobuhide Doi
Researcher at Keio University
Publications - 77
Citations - 2045
Nobuhide Doi is an academic researcher from Keio University. The author has contributed to research in topics: mRNA display & Peptide sequence. The author has an hindex of 25, co-authored 73 publications receiving 1864 citations. Previous affiliations of Nobuhide Doi include Mitsubishi & Hokkaido University.
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Journal ArticleDOI
STABLE: protein-DNA fusion system for screening of combinatorial protein libraries in vitro.
Nobuhide Doi,Hiroshi Yanagawa +1 more
TL;DR: This method, named ‘STABLE’ (STA‐biotin linkage in emulsions), should be useful for rapid in vitro evolution of proteins and for ligand‐based selection of cDNA libraries.
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DNA display for in vitro selection of diverse peptide libraries
TL;DR: DNA display is expected to be useful for rapid screening of a wide variety of peptide ligands for corresponding receptors by avoiding the use of bacterial cells and RNA tags.
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Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis
TL;DR: The results suggest that rearrangement of biosynthetic genes in the order of the metabolic pathway by the OGAB method could be a useful approach for metabolic engineering.
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In vitro evolution of single-chain antibodies using mRNA display
Isao Fukuda,Kanehisa Kojoh,Noriko Tabata,Nobuhide Doi,Hideaki Takashima,Etsuko Miyamoto-Sato,Hiroshi Yanagawa +6 more
TL;DR: In this article, the authors describe the application of the in vitro virus mRNA display method, which involves covalent linkage of an in vitro-synthesized antibody (phenotype) to its encoding mRNA (genotype) through puromycin, for in vitro evolution of single-chain Fv (scFv) antibody fragments.
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Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution
Nobuhide Doi,Hiroshi Yanagawa +1 more
TL;DR: A general method of creating entirely new molecular‐recognition sites on GFPs by inserting a protein domain containing a desired molecular‐binding site into a surface loop of GFP is described.