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Nuno Carinhas
Researcher at Universidade Nova de Lisboa
Publications - 25
Citations - 996
Nuno Carinhas is an academic researcher from Universidade Nova de Lisboa. The author has contributed to research in topics: Metabolic flux analysis & Metabolism. The author has an hindex of 15, co-authored 25 publications receiving 855 citations.
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Cell density effect in the baculovirus-insect cells system: a quantitative analysis of energetic metabolism.
TL;DR: Interestingly, the energetic status of the culture correlated with the decrease in cellular production of baculovirus, meaning that there is room for process optimization through the application of metabolic engineering techniques.
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In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures.
Ana P. Teixeira,Carla A.M. Portugal,Nuno Carinhas,João M. L. Dias,João P. Crespo,Paula M. Alves,Manuel J.T. Carrondo,Rui Oliveira +7 more
TL;DR: 2D fluorometry constitutes a reliable methodology for on‐line monitoring of viable cells and recombinant protein concentrations in mammalian cell cultures and two validation strategies were used to evaluate the predictive capacity of the developed PLS models.
Journal ArticleDOI
Improving baculovirus production at high cell density through manipulation of energy metabolism.
Nuno Carinhas,Vicente Bernal,Francisca Monteiro,Manuel J.T. Carrondo,Rui Oliveira,Paula M. Alves +5 more
TL;DR: The work herein presented describes a combined experimental and modelling effort to quantify and environmentally manipulate the metabolism of Spodoptera frugiperda cells, targeting high cell density production of baculovirus vectors with potential application in human gene therapy.
Journal ArticleDOI
Metabolic signatures of GS‐CHO cell clones associated with butyrate treatment and culture phase transition
Nuno Carinhas,Tiago M. Duarte,Laura C. Barreiro,Manuel J.T. Carrondo,Paula M. Alves,Ana P. Teixeira +5 more
TL;DR: This study shows that early butyrate treatment has a marked effect on sustaining high nutrient consumption along culture time, being more pronounced during the stationary phase when extra energy generation and biosynthetic activity is fueled to increase IgG formation.
Journal ArticleDOI
Metabolic responses of CHO cells to limitation of key amino acids
Tiago M. Duarte,Nuno Carinhas,Laura C. Barreiro,Manuel J.T. Carrondo,Paula M. Alves,Ana P. Teixeira +5 more
TL;DR: Asparagine is confirmed to be essential for these GS‐CHO cells as the main source of intracellular nitrogen as well as having an important anaplerotic role in TCA cycle activity.