A new method for the ablation of undesirable tissue such as cells of a cancerous or non-cancerous tumor is disclosed. It involves the placement of electrodes into or near the vicinity of the undesirable tissue through the application of electrical pulses causing irreversible electroporation of the cells throughout the entire area of the undesirable tissue. The electric pulses irreversibly permeate the cell membranes, thereby invoking cell death. The irreversibly permeabilized cells are left in situ and are removed by the body immune system. The amount of tissue ablation achievable through the use of irreversible electroporation without inducing thermal damage is considerable.

Tissue ablation with irreversible electroporation

The application of strong electric fields in water and organic liquids has been studied for several years, because of its importance in electrical transmission processes and its practical applications in biology, chemistry, and electrochemistry. More recently, liquid-phase electrical discharge reactors have been investigated, and are being developed, for many environmental applications, including drinking water and wastewater treatment, as well as, potentially, for environmentally benign chemical processes. This paper reviews the current status of research on the application of high-voltage electrical discharges for promoting chemical reactions in the aqueous phase, with particular emphasis on applications to water cleaning.

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Electrohydraulic Discharge and Nonthermal Plasma for Water Treatment

Irreversible electroporation (IRE) is a new tissue ablation technique in which micro to millisecond electrical pulses are delivered to undesirable tissue to produce cell necrosis through irreversible cell membrane permeabilization. IRE affects only the cell membrane and no other structure in the tissue. The goal of the study is to test our IRE tissue ablation methodology in the pig liver, provide first experience results on long term histopathology of IRE ablated tissue, and discuss the clinical implications of the findings. The study consists of: a) designing an IRE ablation protocol through a mathematical analysis of the electrical field during electroporation; b) using ultrasound to position the electroporation electrodes in the predetermined locations and subsequently to monitor the process; c) applying the predetermined electrotroporation pulses; d) performing histolopathology on the treated samples for up to two weeks after the procedure; and e) correlating the mathematical analysis, ultrasound data...

https://journals.sagepub.com/doi/pdf/10.1177/153303460700600106

Irreversible electroporation: a new ablation modality--clinical implications.

Cellulosic paper and oil insulation in a transformer degrade at higher operating temperatures. Degradation is accelerated in the presence of oxygen and moisture. Power transformers being expensive items need to be carefully monitored throughout their operation. Well established time-based maintenance and conservative replacement planning is not feasible in a current market driven electricity industry. Condition based maintenance and online monitoring are now gaining importance. Currently there are varieties of chemical and electrical diagnostic techniques available for insulation condition monitoring of power transformers. This paper presents a description of commonly used chemical diagnostics techniques along with their interpretation schemes. A number of new chemical techniques are also described in this paper. A number of electrical diagnostic techniques have gained exceptional importance to the utility professionals. Among these techniques polarisation/depolarisation current measurement, return voltage measurement and frequency domain dielectric spectroscopy at low frequencies are the most widely used. This paper describes analyses and interpretation of these techniques for transformer insulation condition assessment.

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Review of modern diagnostic techniques for assessing insulation condition in aged transformers

An important tool for improving the reliability of HV insulation systems are partial discharge (PD) measurements. The interpretation of such measurements aims at extracting from the measured data information about insulation defects which then are used for estimating the risk of insulation failure of the equipment. Because the physical understanding of PD has made substantial progress in the last decade, it can now be exploited to support interpretation. In this paper a concept is presented which merges the available physical knowledge about various PD types into a generalized model which can be applied to arbitrary insulation defects. This approach will be restricted to PD of the streamer type in gases and at gas-insulator interfaces which cover a large fraction of the cases encountered in technical insulation systems. The generalized model allows us to derive approximate relations between defect characteristics, insulation design parameters and test conditions on one side, and measurable PD characteristics on the other. The inversion of these relations yields rules for extracting defect information from the PD data. The application of the generalized model is illustrated by two simple examples, namely, spherical voids in an insulator and electrode protrusions in SF/sub 6/. >

A generalized approach to partial discharge modeling

The fundamental theory of the UHF method for detecting partial discharge (PD) in gas insulated substations (GIS) is presented. The effects of position, size, current amplitude and pulse shape of the PD source on the UHF signal can be predicted using this theory. Excitation of propagating electromagnetic waves by a PD current pulse within the coaxial waveguides formed by GIS components is explained by making use of dyadic Green's functions for the electric fields of propagating modes. Experiments with a coaxial test chamber are used to verify the theoretical predictions, and comparisons are made between measured and simulated UHF signals. Some implications for the UHF measurement of PD are discussed, together with positioning and sensitivity requirements for UHF couplers. A scheme is proposed for standardizing PD measurements made using the UHF technique.

The excitation of UHF signals by partial discharges in GIS

The effects of high-intensity pulsed-light emissions of high or low UV content on the survival of predetermined populations of Listeria monocytogenes, Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Bacillus cereus, and Staphylococcus aureus were investigated. Bacterial cultures were seeded separately on the surface of tryptone soya-yeast extract agar and were reduced by up to 2 or 6 log10 orders with 200 light pulses (pulse duration, approximately 100 ns) of low or high UV content, respectively (P < 0.001).

Pulsed-Light Inactivation of Food-Related Microorganisms

The lethality of high-intensity pulsed-light emissions from low and high ultraviolet (UV) light sources on predetermined microbial populations has been investigated. Prior to treatment, the bacterial enteropathogens Bacillus cereus, Escherichia coli, and Salmonella enteritidis and the food-spoilage fungi Aspergillus niger and Fusarium culmorum were seeded separately onto the surface of either tryptone soya yeast extract or malt extract agar plates. Prescribed microbial population densities were applied to the test media and these samples were exposed to one of two light sources. These were low-pressure, xenon filled, flash lamps that produced either high or low UV intensities. They were operated in pulsed mode, being driven by a stacked Blurnlein table generator. Microbial samples were treated by exposure to different numbers of light pulses. The treated bacterial populations were reduced by /spl sim/8 log orders after 1000 light-pulses of the higher UV intensity light and the fungal counts had a corresponding reduction of 4.5 log orders. The fungus, Aspergillus niger, was shown to be significantly more resistant in spore form to the intense UV light compared with Fusarium culmorum. This resistance has been attributed to the high level of UV absorbance associated with the dark pigment present in A. niger. The pulsed light source of lower UV intensity was shown to be significantly less effective in reducing microbial populations.

Inactivation of food-borne enteropathogenic bacteria and spoilage fungi using pulsed-light

A defect such as a free metallic particle or stress-raising edge on an electrode could lead to breakdown in a gas insulated substation (GIS), but may be detected by the partial discharges it generates. Of the various detection means available, most success has been achieved with either acoustic transducers to detect sound waves from the discharge, or electrical couplers to pick up the ultra-high-frequency (UHF) signals generated inside the GIS chambers. All new GIS in the UK are fitted with internal couplers for UHF monitoring, and two examples of continuous monitoring schemes on 400 kV GIS at nuclear power stations are described. Further developments, which are already well advanced, include specifying and measuring the coupler characteristics, making use of expert systems to interpret the UHF discharge data, and extending the monitoring system to include the performance of circuit breakers and other equipment. >

Partial discharge diagnostics for gas insulated substations

The effects of high intensity light emissions, produced by a novel pulsed power energization technique (PPET), on the survival of bacterial populations of verocytotoxigenic Escherichia coli (serotype 0157:H7) and Listeria monocytogenes (serotype 4b) were investigated. Using this PPET approach, many megawatts (MW) of peak electrical power were dissipated in the light source in an extremely short energization time (about 1 μs). The light source was subjected to electric field levels greater than could be achieved under conventional continuous operation, which led to a greater production of the shorter bacteriocidal wavelengths of light. In the exposure experiments, pre-determined bacterial populations were spread onto the surface of Trypone Soya Yeast Extract Agar and were then treated to a series of light pulses (spectral range of 200–530 nm) with an exposure time ranging from 1 to 512 μs. While results showed that as few as 64 light pulses of 1 μs duration were required to reduce E. coli 0157:H7 populations by 99·9% and Listeria populations by 99%, the greater the number of light pulses the larger the reduction in cell numbers (P < 0·01). Cell populations of E. coli 0157:H7 and Listeria were reduced by as much as 6 and 7 log10 orders at the upper exposure level of 512 μs, respectively. Survival data revealed that E. coli 0157:H7 was less resistant to the lethal effects of radiation (P < 0·01). These studies have shown that pulsed light emissions can significantly reduce populations of E. coli 0157:H7 and L. monocytogenes on exposed surfaces with exposure times which are 4–6 orders of magnitude lower than those required using continuous u.v. light sources.

O. Farish

Papers

The excitation of UHF signals by partial discharges in GIS

Pulsed-Light Inactivation of Food-Related Microorganisms

Inactivation of food-borne enteropathogenic bacteria and spoilage fungi using pulsed-light

Partial discharge diagnostics for gas insulated substations

Light inactivation of food-related pathogenic bacteria using a pulsed power source.