The present invention comprises a method for preparing a commercial beta -amylase product from whole or at least partially dehusked barley grain by extracting the grain with water. The water may optionally contain a reducing agent. The invention further comprises the purification, concentration and stabilization of the beta -amylase solution and the resultant commerical product.

Process for the extraction of beta -amylase from barley grains

New and useful methods of producing stabilized enzyme antibody conjugates are disclosed which are particularly useful in forming multi-layer immunoassay test devices. In particular, the invention concerns the formation of a manganese ion and enzyme-antibody conjugate in aqueous solution and drying the solution to produce a dry stabilized enzyme-antibody conjugate. Further, this stabilized enzyme-antibody conjugate can be formed on a continuous web and dried in a heat tunnel. This continuous manufacturing process allows for the more efficient production of multi-layer test strips.

Salt stabilization of antibody-enzyme conjugates heat-dried into paper

The present invention comprises a method for preparing a commercial β-amylase product from whole or at least partially dehusked barley grain by extracting the grain with water. The water may optionally contain a reducing agent. The invention further comprises the purification, concentration and stabilization of the β-amylase solution and the resultant commerical product.

Process for the extraction of β-amylase from barley grains

The present invention relates to a method for obtaining a preparation of β-amylases from the soluble fraction of starch plants, characterized in that the soluble fraction of starch plants is selected from the group consisting of the soluble fractions of wheat, pea, broad bean, horse bean, rice, barley, rye, buckwheat, potato and sweet potato, and preferably of wheat and barley, a clarification of said soluble fractions is carried out in such a way as to remove therefrom the insoluble substances and the colloids and, optionally, an ultrafiltration of said clarified soluble fractions is carried out in such a way as to obtain an ultrafiltration retentate containing the concentrated β-amylase and an ultrafiltration permeate, said ultrafiltration retentate containing the concentrated β-amylase is diafiltered and the resulting β-amylase is recovered.

Method for obtaining a preparation of beta-amylases from the soluble fractions of starch plants

A process for enzymatically treating a plant, such as a fruit, vegetable, or other plant parts (e.g., leaves, stems, roots, tubers, etc.), is disclosed. The process of the invention combines enzyme treatment of the plant with filtration to obtain a permeate and a retentate. The liquefaction enzyme used to treat the plant is maintained in an active state during the filtration step.

Process for enzymatic treatment and filtration of a plant and products obtainable thereby

An aqueous solution containing β-amylase obtained by extraction of β-amylase-containing plants with water or a buffer solution can be efficiently concentrated and purified at a high temperature by membrane separation without inactivation of β-amylase and putrefaction of the aqueous solution by incorporating a divalent or trivalent metal ion into the aqueous solution and adjusting the pH of the aqueous solution to about 4.5 to 8.0.

Osamu Shibata

Papers

Stabilization of β-amylase in aqueous medium