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Author

Otto Folin

Other affiliations: McLean Hospital
Bio: Otto Folin is an academic researcher from Harvard University. The author has contributed to research in topics: Urine & Uric acid. The author has an hindex of 42, co-authored 116 publications receiving 9832 citations. Previous affiliations of Otto Folin include McLean Hospital.
Topics: Urine, Uric acid, Urea, Creatine, Creatinine


Papers
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Journal ArticleDOI
TL;DR: In this article, the authors summarized research into the existing methods for the quantitative determination of tyrosine and tryptophane in proteins, including the Folin-Looney method, which is based on reaction of a phosphotungstic phosphomolybdic acid in a phenol solution.

2,527 citations

Journal ArticleDOI
Otto Folin1, Hsien Wu1

1,101 citations

Journal ArticleDOI
Otto Folin1, W. Denis1
TL;DR: It is found that during this evaporation of faintly alkaline urine a considerable proportion of the phenol is oxidized, thus producing results much below the truth.

401 citations


Cited by
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Journal Article
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.

289,852 citations

Book ChapterDOI
TL;DR: Aggregate analysis of this type is an important supplement to and often more informative than reems of data difficult to summarize from various techniques, such as high-performance liquid chromatography (HPLC) that separate a large number of individual compounds.
Abstract: Publisher Summary This chapter discusses the analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Analyses of the Folin-Ciocalteu (FC) type are convenient, simple, and require only common equipment and have produced a large body of comparable data. Under proper conditions, the assay is inclusive of monophenols and gives predictable reactions with the types of phenols found in nature. Because different phenols react to different degrees, expression of the results as a single number—such as milligrams per liter gallic acid equivalence—is necessarily arbitrary. Because the reaction is independent, quantitative, and predictable, analysis of a mixture of phenols can be recalculated in terms of any other standard. The assay measures all compounds readily oxidizable under the reaction conditions and its very inclusiveness allows certain substances to also react that are either not phenols or seldom thought of as phenols (e.g., proteins). Judicious use of the assay—with consideration of potential interferences in particular samples and prior study if necessary—can lead to very informative results. Aggregate analysis of this type is an important supplement to and often more informative than reems of data difficult to summarize from various techniques, such as high-performance liquid chromatography (HPLC) that separate a large number of individual compounds .The predictable reaction of components in a mixture makes it possible to determine a single reactant by other means and to calculate its contribution to the total FC phenol content. Relative insensitivity of the FC analysis to many adsorbents and precipitants makes differential assay—before and after several different treatments—informative.

14,046 citations

Journal ArticleDOI
TL;DR: In this paper, the reliability of the various Somogyi-Shaffer-Hartmann (SHH) copper reagents for glucose determination in biological material has been established, which can be accomplished by omission of the iodide and iodate in their preparation, since these interfere with the molybdate color reagents.

10,346 citations

Journal ArticleDOI
TL;DR: A simple method based on a linear log-log protein standard curve is presented to permit rapid and totally objective protein analysis using small programmable calculators.

8,197 citations