Protein Measurement with the Folin Phenol Reagent

Publisher Summary This chapter discusses the analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Analyses of the Folin-Ciocalteu (FC) type are convenient, simple, and require only common equipment and have produced a large body of comparable data. Under proper conditions, the assay is inclusive of monophenols and gives predictable reactions with the types of phenols found in nature. Because different phenols react to different degrees, expression of the results as a single number—such as milligrams per liter gallic acid equivalence—is necessarily arbitrary. Because the reaction is independent, quantitative, and predictable, analysis of a mixture of phenols can be recalculated in terms of any other standard. The assay measures all compounds readily oxidizable under the reaction conditions and its very inclusiveness allows certain substances to also react that are either not phenols or seldom thought of as phenols (e.g., proteins). Judicious use of the assay—with consideration of potential interferences in particular samples and prior study if necessary—can lead to very informative results. Aggregate analysis of this type is an important supplement to and often more informative than reems of data difficult to summarize from various techniques, such as high-performance liquid chromatography (HPLC) that separate a large number of individual compounds .The predictable reaction of components in a mixture makes it possible to determine a single reactant by other means and to calculate its contribution to the total FC phenol content. Relative insensitivity of the FC analysis to many adsorbents and precipitants makes differential assay—before and after several different treatments—informative.

Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent

A photometric adaptation of the somogyi method for the determination of glucose

http://www.sciencemadness.org/talk/files.php?pid=78294&aid=1880

A simplification of the protein assay method of Lowry et al. which is more generally applicable

Notes on sugar determination

On tyrosine and tryptophane determinations in proteins

A system of blood analysis

http://www.jbc.org/content/12/2/239.full.pdf

Otto Folin

Papers

On tyrosine and tryptophane determinations in proteins

A system of blood analysis

On phosphotungstic-phosphomolybdic compounds as color reagents

A SYSTEM OF BLOOD ANALYSIS Supplement I. A SIMPLIFIED AND IMPROVED METHOD FOR DETERMINATION OF SUGAR

A colorimetric method for the determination of phenols (and phenol derivatives) in urine