https://aasldpubs.onlinelibrary.wiley.com/doi/pdf/10.1002/hep.1840200611

Prevalence of chronic liver disease in the general population of northern Italy: the Dionysos Study.

Over a century ago, Theodor Boveri paved the way to mechanistic studies linking chromosomal abnormalities to cancer pathogenesis. Since then, theoretical and empirical evidence has been accumulated, supporting a causal role of these events in the aetiology of human cancer. A powerful tool for measurement of chromosomal abnormalities is the cytokinesis-block micronucleus cytome (CBMNcyt) assay. The validation of the micronucleus (MN) as marker of phenotypic susceptibility to cancer has received decisive support from mutagens sensitivity studies, particularly from a recent case-control study on lung cancer, which showed increased frequency of tobacco carcinogen-induced MN, nuclear buds and especially nucleoplasmic bridges in cancer patients (odds ratios of 2.3, 10.0 and 45.5, respectively). Recently, a large international cohort study showed a significant association between MN frequency in healthy subjects and cancer risk. The study assembled data on 6718 individuals from 10 countries (62 980 person-years). Cancers incidence was significantly higher in groups with medium (RR = 1.84; 95% confidence interval: 1.28-2.66) and high MN frequency (RR = 1.53; 95% CI: 1.04-2.25). This study provided preliminary evidence that MN frequency in peripheral blood lymphocytes is predictive of cancer risk, suggesting that increased MN formation is associated with early events in carcinogenesis. These results, in combination with mechanistic evidence, prospected the use of MN frequency in cancer screening programmes. However, issues such as interindividual variability and preventive strategies in high-risk groups need to be further addressed to consolidate these achievements.

/pdf/micronuclei-frequency-in-peripheral-blood-lymphocytes-and-426cqt47a2.pdf

Micronuclei frequency in peripheral blood lymphocytes and cancer risk: evidence from human studies

http://www.medicinabiomolecular.com.br/biblioteca/pdfs/Doencas/do-1138.pdf

Effect of ascorbic acid on bone marrow-derived mesenchymal stem cell proliferation and differentiation.

Reduced oxygen species, mutation, induction and cancer initiation.

Preeclampsia and antioxidant nutrients : decreased plasma levels of reduced ascorbic acid, α-tocopherol, and beta-carotene in women with preeclampsia

The effects of AA and DHA on ATP C+ cell multiplication in vitro were studied by measuring incorporation of 3H thymidine into DNA. The results obtained demonstrate that both AA and DHA have the same effects: they favor cell multiplication at low doses and inhibit it at high doses. Experiments carried out with serial doses of both these substances revealed that AA is more efficient in determining both stimulating and inhibiting effects. The lesser efficiency of DHA may be attributed to its limited stability in culture medium. Studies on the effect of high doses of AA and DHA added to the culture medium in single or fractionated doses revealed that fractionated administration is more efficient in inhibiting cell multiplication than single administration.

https://link.springer.com/content/pdf/10.1007/BF00402472.pdf

Effects of ascorbic and dehydroascorbic acid on the multiplication of tumor ascites cells in vitro

Frequency of sister chromatid exchanges and micronuclei monitored over time in patients with early-stage breast cancer: results of an observational study

Variations in catalase, glutathione peroxidase and superoxide dismutase in regenerating rat liver.

Superoxide dismutase and catalase were demonstrated to favour the multiplication of ascites tumour cells in vitro. It is proposed that these enzymes neutralize the O
 2
 .
 
− and H2O2 that may accumulate in the neoplastic cell and that cell damage occurs because the cellular levels of both enzymes are low.

Cell multiplication of an ascites tumour in the presence of superoxide dismutase and catalase

A study on the capacity of ascorbic acid, reduced glutathione and cysteine to interfere with 3H-7,12-dimethylbenz[a]anthracene (3H-DMBA) binding to DNA in cultured fibroblast-like cells from 11-day-old chick embryos showed that, although the total amount of 3H-DMBA in the treated cells was greater than in the untreated cells, the DNA-bound 3H-DMBA was less. Comparisons between the various experimental groups demonstrated that the greater 3H-DMBA in the ascorbic acid-, reduced glutathione-, and cysteine-treated groups could not be attributed to an initially higher number of cells, nor to a treatment-induced increase in DNA synthesis. It is proposed that the three substances examined inhibit the oxidative degradation of 3H-DMBA, thereby favoring its accumulation within the cell and reducing the formation of DNA-binding metabolites.

Inhibition of the Binding of 7,12-Dimethylbenz[a]anthracene to DNA by Ascorbic Acid, Reduced Glutathione and Cysteine in Chick Embryo Cells Cultured in vitro

P. Guerrieri

Papers

Effects of ascorbic and dehydroascorbic acid on the multiplication of tumor ascites cells in vitro

Frequency of sister chromatid exchanges and micronuclei monitored over time in patients with early-stage breast cancer: results of an observational study

Variations in catalase, glutathione peroxidase and superoxide dismutase in regenerating rat liver.

Cell multiplication of an ascites tumour in the presence of superoxide dismutase and catalase

Inhibition of the Binding of 7,12-Dimethylbenz[a]anthracene to DNA by Ascorbic Acid, Reduced Glutathione and Cysteine in Chick Embryo Cells Cultured in vitro