Showing papers by "Paul F. Greenfield published in 1999"

Towards a fourth generation R&D management model-research networks in knowledge management

Abstract: Research and development (R&D) management is increasingly about managing knowledge rather than simply managing its generation. Better management of knowledge is a key success factor for industry competitiveness through continuous innovation. R&D management processes developed in the past, which can be described as the first, second, or third generation models, deal with concepts, techniques and tools for managing research as an investment portfolio of the firm. These models focus on the creation and diffusion of knowledge internal to the firm. Management of R&D is also about managing knowledge external to the firm and it involves the management of complementary skills, technological dependencies, and knowledge transfers across research links. This paper addresses the concept of research management as the generation of intellectual capital, which drives future businesses and new products. It examines, as a case study, research management practices employed by biotechnology and pharmaceutical research groups in industries and universities and outlines a fourth generation approach to managing research.

Optimized production of recombinant bluetongue core-like particles produced by the baculovirus expression system.

Abstract: The baculovirus-expression vector system (BEVS) has been widely used for the experimental production of many human and animal single- and multi-unit vaccines, heterologous proteins, and viral insecticides. In this work, the production of recombinant bluetongue virus core-like particles (CLPs), using Sf9 cells in shaker-suspension culture with the SF900 II medium (GIBCO, NY), has been studied. This system involved the simultaneous production of two proteins, VP7 and VP3, and was shown to achieve high volumetric productivities. The key parameters of the time of infection (TOI), and the multiplicity of infection (MOI) were studied. The results show that the peak-volumetric yields and cell-specific yields achieved using low MOIs at low-cell densities were the same as those obtained following infections with a high MOI at high-cell densities. This work establishes the feasibility of using low MOIs in the baculovirus system to produce complex multiprotein particles. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 600–604, 1999.