P
Paul K. Nakane
Researcher at Stanford University
Publications - 7
Citations - 7502
Paul K. Nakane is an academic researcher from Stanford University. The author has contributed to research in topics: Vitamin & Anterior pituitary. The author has an hindex of 7, co-authored 7 publications receiving 7445 citations.
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Journal ArticleDOI
Periodate-lysine-paraformaldehyde fixative a new fixative for immunoelectron microscopy
Ian W. Mclean,Paul K. Nakane +1 more
TL;DR: Using this fixative and the peroxidase-labeled antibody technique, basement membrane antigen was localized within the cisternae of endoplasmic reticulum of parietal yolk sac cells and in extracellular basement membranes with adequate tissue preservation, a task which has not been successfully accomplished by conventional fixatives.
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Peroxidase-labeled antibody. A new method of conjugation
Paul K. Nakane,Akira Kawaoi +1 more
TL;DR: A new method of conjugating horseradish peroxidase with proteins was developed by oxidizing the carbohydrate moiety with sodium periodate and bound to free amino groups of proteins unidirectionally at high efficiencies.
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Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat.
TL;DR: The peroxidase-labeled antibody method was modified to localize multiple tissue antigens in a single histologic section, using substrates that develop reaction products of different colors to identify the antigenic sites.
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Classifications of anterior pituitary cell types with immunoenzyme histochemistry
TL;DR: Peroxidase-labeled antibody method was used to localize the six hormones of the anterior pituitary gland of male rats both at the light and electron microscopic levels, and gonadotropic cells may be identified but their hormone content cannot be determined.
Journal ArticleDOI
Localization of tissue antigens on the ultrathin sections with peroxidase-labeled antibody method.
Yasuyuki Kawarai,Paul K. Nakane +1 more
TL;DR: Peroxidase-labeled antibody method was used to localize tissue antigens directly on ultrathin sections of methacrylate-embedded anterior pituitary gland of the rat and totally eliminates the problem of penetration of antisera through tissues and permits better immunologically controlled experiments by using serially sectioned materials.