Periodate-lysine-paraformaldehyde fixative a new fixative for immunoelectron microscopy
Ian W. Mclean,Paul K. Nakane +1 more
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Using this fixative and the peroxidase-labeled antibody technique, basement membrane antigen was localized within the cisternae of endoplasmic reticulum of parietal yolk sac cells and in extracellular basement membranes with adequate tissue preservation, a task which has not been successfully accomplished by conventional fixatives.Abstract:
A new fixative which primarily stabilizes carbohydrate moieties was developed for immunoelectron microscopy. It contains periodate, lysine and paraformaldehyde. Theoretically, the carbohydrates are oxidized by periodate and cross-linked by lysine. The fixative can preserve antigenicity as well as paraformaldehyde and ultrastructure as well as glutaraldehyde. Using this fixative and the peroxidase-labeled antibody technique, basement membrane antigen was localized within the cisternae of endoplasmic reticulum of parietal yolk sac cells and in extracellular basement membranes with adequate tissue successfully accomplished by conventionalread more
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References
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Peroxidase-labeled antibody. A new method of conjugation
Paul K. Nakane,Akira Kawaoi +1 more
TL;DR: A new method of conjugating horseradish peroxidase with proteins was developed by oxidizing the carbohydrate moiety with sodium periodate and bound to free amino groups of proteins unidirectionally at high efficiencies.
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TL;DR: The principles of microscopy principles in light microscopy scanning techniques in electron microscopy and their applications in transmission/scanning and digital holographic microscopy are explained.
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Theoretical and practical aspects of glutaraldehyde fixation.
TL;DR: The many structures put forward for glutaraldehyde, and the purification of the commercial material for chemical, histological and histochemical studies are considered, together with the physical changes in the proteins during the reactions.